Question
- Why must a cell be made 'competent’ in biotechnology experiments? How does calcium ion help in doing so?
- State the role of ‘biolistic gun’ in biotechnology experiments.
✓
Answer
- To take up the (hydrophilic) DNA from the external medium.
- Divalent calcium ions increase the efficiency of DNA entering the cell through pores in the cell wall.
- To introduce alien DNA into the plant cell by bombarding them with high-velocity microparticles. (gold or tungsten coated with DNA)
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