Question
- Write the palindromic nucleotide sequence for the following DNA segment:
5' - GAATTC - 3'
- Name the restriction endonuclease that recognises this sequence.
- How are 'sticky ends' produced? Mention their role.
✓
Answer
- 5' - GAATTC - 3'
3' - CTTAAG - 5'
- EcoRI is the restriction endonuclease that recognizes this palindrome.
- When the restriction enzyme cuts the DNA strands a little away from the centre of the palindromic sequence, between the same two bases on both the strands, sticky ends are produced.
- The stickiness of the ends facilitates the action of the enzyme DNA-ligase.
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