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Molecular Basis of Inheritance — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYMolecular Basis of Inheritance3 Marks
Question
You are repeating the Hershey-Chase experiment and are provided with two isotopes: 32P and 15N (in place of 35S in the original experiment). How do you expect your results to be different?
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Answer

Use of 15N will be inappropriate because method of detection of 35P and 15N is different (32P being a radioactive isotope while 15N is not radioactive but is the heavier isotope of nitrogen). Even if 15N was radioactive then its presence would have been detected both inside the cell (15N incorporated as nitrogenous base in DNA) as well as in the supernatant because 15N would also get incorporated in amino group of amino acids in proteins). Hence, the use of 15N would not give any conclusive results.

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