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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
You have chosen a plasmid as vector for cloning your gene. However this vector plasmid lacks a selectable marker. How would it affect your experiment?
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Answer

In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector and introduced inside the host cell (transformation).
Since, not all the cells gets transformed with the recombinant/ plasmid DNA, in the absence of selectable marker, it will be difficult to distinguish between transformants and non transformants. Because the role of selectable marker is in the selection of transformants.

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