Agarose gel is extracted from:
- ✓Fungus
- BSea weeds
- CHydrilla
- DWheat
Answer: A.
View full solution →Question types
Biotechnology: Principles and Processes question types
115 questions across 6 question groups — pick any mix to generate a BIOLOGY paper with step-by-step answer keys.
115
Questions
6
Question groups
5
Question types
01
M.C.Q [1M]
33 Q→02Fill in blanks.
11 Q→031 Marks Question
29 Q→04Short Answer Type Questions [2M]
20 Q→053 Marks Question
15 Q→06Long Answer Type Questions [5M]
7 Q→Sample Questions
Biotechnology: Principles and Processes questions
One sample from each question group in this chapter. Select any group above to see the full set with answer keys.
Enzyme which works by cutting DNA into segments:
- ✓DNA ligase
- BRestriction enzyme
- CDNA polymerase
- DProtease enzyme
Answer: A.
View full solution →
The following statements describe the characteristics of restriction endonuclease enzyme. Choose the wrong statement:
- AThis enzyme identifies a specific palindromic nucleotide sequence on DNA.
- BThis enzyme on DNA at the identified location, cuts the DNA molecule.
- ✓This enzyme joins the DNA at specific sites and cuts only one of the two strands.
- DThis enzyme cuts the sugar phosphate cord at specific sites on each strand.
Answer: C.
View full solution →Which technique is used to isolate the cut pieces of DNA by restriction endonuclease technique?
- ABioreactor
- ✓Electrophoresis
- CBiologic
- DMicroinjection
Answer: B.
View full solution →The cutting of DNA at specific sites became possible with the discovery of _________ the enzyme called as molecular scissors.
The process of separation and purification of expressed proteins before sending them to the market is called _________.
In transgenic organism, expression of the transgene in the target tissue is determined by a _________.
pBR322 vector carry the resistance gene for _________.
The vector used for gene cloning in most of the dicotyledonous plant is _________ and in animal cell is _________.
Write one method of introducing foreign DNA into host cells.
What are transgenic animals?
What is the definition of biotechnology given by the European Biotechnology Association?### Define biotechnology.
How can separated DNA fragments be visualized by gel electrophoresis?
Describe the principle of separating DNA fragments by gel electrophoresis technique.
Explain any one feature of cloning conductor.
Explain any one feature necessary for cloning in a vector.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Do eukaryotic cells have restriction endonucleases? Justify your answer correct.
Make a chart (with diagrammatic representation) showing restriction enzyme, the substrate DNA on which it acts on, the site at which it cuts DNA the product.
Draw a diagram of E.coli cloning vector pBR322.
Draw the named diagram of sparged stirred tank bioreacter.
Can you recall meiosis and indicate at what stage a recombinant DNA is made?
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks.
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following the base-pair rules.
find out how to distinguish between: RNA and DNA
Explain : Restriction Enzymes and DNA
Describe the following: Bioreactor
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
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