MCQ 11 Mark
Kary $B$ Mullis got Nobel prize for discovering:
AnswerCorrect option: B. Polymerase chain reaction
View full question & answer→MCQ 21 Mark
The most important feature in a plasmid to be used as a vector is:
AnswerCorrect option: A. Origin of replication $($ori$).$
View full question & answer→MCQ 31 Mark
There is a restriction endonuclease called Eco Ri. What does $'CO\ '$ part of it stands for:
View full question & answer→MCQ 41 Mark
Transfer of $\text{DNA}$ bands from agarose gel to nitrocellulose or nylon membrane is:
View full question & answer→MCQ 51 Mark
Gene gun can introduce genes into cells with the help of:
AnswerIn the gene gun method, microscopic pellets of gold or tungsten are coated with the transgene fragments and shot into the plant cells or tissues at high velocity.
View full question & answer→MCQ 61 Mark
Amplification of gene of interest by using $\text{PCR}$ may go upto:
- A
$0.1$ million
- B
$1.0$ million
- ✓
$1.0$ billion
- D
$1.0$ trillion
AnswerCorrect option: C. $1.0$ billion
View full question & answer→MCQ 71 Mark
An enzyme catalysing the removal of nucleotides from the ends of $\text{DNA}$ is:
- A
- ✓
- C
$\text{DNA}$ ligase.
- D
Hind$-II.$
View full question & answer→MCQ 81 Mark
Recombinant $\text{DNA}$ is obtained by cleaving the pro$-\text{DNA}$ by:
- A
- B
- C
- ✓
Restriction endonuclease.
AnswerCorrect option: D. Restriction endonuclease.
View full question & answer→MCQ 91 Mark
At which of the following stage after fermentation, the desired product is screened and purified?
MCQ 101 Mark
Plasmids are suitable vectors for gene clon$-$ing because:
AnswerCorrect option: B. These are small circular $\text{DNA}$ molecules with their own replication origin site.
View full question & answer→MCQ 111 Mark
Bacteria protect themselves from viruses by fragmenting viral $\text{DNA}$ with:
View full question & answer→MCQ 121 Mark
'Restriction' in Restriction enzyme refers to:
AnswerCorrect option: C. Prevention of the multiplication of bacteriophage in bacteria.
View full question & answer→MCQ 131 Mark
For transformation with recombinant $\text{DNA}$ the bacterial cells must first be made ‘competent’ which means:
AnswerCorrect option: C. Increase efficiency with which $\text{DNA}$ enters the bacterium.
View full question & answer→MCQ 141 Mark
Enzyme required for polymerase chain reaction $\text{(PCR)}$ is:
- A
$\text{RNA}$ polymerase
- B
- ✓
- D
View full question & answer→MCQ 151 Mark
The cutting out of separated bands of $\text{DNA}$ from the agarose gel is called:
View full question & answer→MCQ 161 Mark
In which field, application of biotechnology occurs?
AnswerApplication of biotechnology occurs in agriculture, biomedicine and environmental field.
View full question & answer→MCQ 171 Mark
Restriction endonucleases are useful in:
AnswerCorrect option: C. Both $A$ and $B$
View full question & answer→MCQ 181 Mark
Restriction enzymes are isolated chiefly from?
MCQ 191 Mark
Genetic engineering is possible, because:
AnswerCorrect option: D. Restriction endonucleases purified from bacteria can be used in vitro.
View full question & answer→MCQ 201 Mark
The $\text{DNA}$ polymerase enzyme used in $\text{PCR}$ is obtained from:
- ✓
- B
- C
Agrobacterium tumefaciens.
- D
View full question & answer→MCQ 211 Mark
The cutting of $\text{DNA}$ at specific locations became possible with the discovery of:
View full question & answer→MCQ 221 Mark
Excision and insertion of a gene is called.
MCQ 231 Mark
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
AnswerCorrect option: B. A continuous culture system.
View full question & answer→MCQ 241 Mark
Which of the following statements does not hold true for restriction enzyme?
AnswerCorrect option: D. It is isolated from viruses.
View full question & answer→MCQ 251 Mark
Which of the following steps are catalysed by Taq polymerase in a $\text{PCR}$ reaction?
- ✓
Extension of primer end on the template $\text{DNA}.$
- B
- C
Annealing of primers to template $\text{DNA}$
- D
Denaturation of template $\text{DNA}$
AnswerCorrect option: A. Extension of primer end on the template $\text{DNA}.$
View full question & answer→MCQ 261 Mark
Which one of the following hydrolyzes internal phosphodiester bonds in a polynucleotide chain?
MCQ 271 Mark
The process of replication in plasmid $\ce{DNA},$ other than initiation, is controlled by:
View full question & answer→MCQ 281 Mark
In agarose gel electrophoresis?
AnswerCorrect option: D. Ethidium bromide can be used to visualize the $\text{DNA}.$
View full question & answer→MCQ 291 Mark
While isolating $\text{DNA}$ from bacteria, which of the following enzymes is not used?
View full question & answer→MCQ 301 Mark
Which of the following is known as specific molecular scissors?
- A
- B
- ✓
Restriction endonuclease.
- D
$\ce{DNA}$ polymerase.
AnswerCorrect option: C. Restriction endonuclease.
View full question & answer→MCQ 311 Mark
A bacterial cell was transformed with a recombinant $\text{DNA}$ that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
- A
Amino acid codons for humans and bacteria are different.
- B
Human protein is formed but degraded by bacteria.
- ✓
Human gene may have intron which bacteria cannot process.
- D
AnswerCorrect option: C. Human gene may have intron which bacteria cannot process.
View full question & answer→MCQ 321 Mark
The restriction enzymes are used in genetic engineering, because:
- A
They can degrade harmful proteins.
- B
They can join different $\text{DNA}$ fragments.
- ✓
They can cut $\text{DNA}$ at spe cific base sequence.
- D
They are nucleases that cut $\text{DNA}$ at variable sites.
AnswerCorrect option: C. They can cut $\text{DNA}$ at spe cific base sequence.
View full question & answer→MCQ 331 Mark
The polymerase chain reaction is a technique used for?
AnswerCorrect option: A. Amplification of $\text{DNA}$
Polymerase chain reaction is a process in which multiple copies of $\text{DNA}$ of interest is synthesised invitro. The process involves three steps Denaturation, Annealing, Extension
View full question & answer→MCQ 341 Mark
Reaction of $1989$ was:
- A
Nicking by restriction endonucleases
- ✓
Polymerase chain reaction
- C
- D
AnswerCorrect option: B. Polymerase chain reaction
View full question & answer→MCQ 351 Mark
The term 'molecular scissors' generally refers to:
- ✓
$\text{DNA}$ polymerases
- B
$\text{RNA}$ polymerases
- C
Restriction endonucleases
- D
$\text{DNA}$ ligases
AnswerCorrect option: A. $\text{DNA}$ polymerases
View full question & answer→MCQ 361 Mark
Which of the following is a plasmid?
- ✓
$\text{pBR 322}$
- B
$\text{Bam HI}$
- C
$\text{Sal I}$
- D
$\text{Eco RI }$
AnswerCorrect option: A. $\text{pBR 322}$
View full question & answer→MCQ 371 Mark
Significance of 'heat shock' method in bacterial transformation is to facilitate:
- A
Binding of $\text{DNA}$ to the cell wall.
- B
Uptake of $\text{DNA}$ through membrane transport proteins.
- ✓
Uptake of $\text{DNA}$ through transient pores in the bacterial cell wall.
- D
Expression of antibiotic resistance gene.
AnswerCorrect option: C. Uptake of $\text{DNA}$ through transient pores in the bacterial cell wall.
View full question & answer→MCQ 381 Mark
One of the methods of which $\text{DNA}$ cannot be transferred to the host cell is by:
- A
- B
- C
Disarmed pathogen vectors
- ✓
Polymerase chain reaction
AnswerCorrect option: D. Polymerase chain reaction
View full question & answer→MCQ 391 Mark
Which of the following steps are catalysed by Taq polymerase in a $\text{PCR}$ reaction?
- A
Denaturation of template $\text{DNA}.$
- B
Annealing of primers to template $\text{DNA}.$
- ✓
Extension of primer end on the template $\text{DNA}.$
- D
AnswerCorrect option: C. Extension of primer end on the template $\text{DNA}.$
View full question & answer→MCQ 401 Mark
Which of the following statements is not true for stirred tank fermentation?
MCQ 411 Mark
During isolation of $\text{DNA}$ addition of which of the following causes precipitation of purified $\text{DNA}?$
- ✓
- B
- C
$\text{DNA}$ polymerase.
- D
View full question & answer→MCQ 421 Mark
The technique in which foreign $\text{DNA}$ is precipitated over surface of metal particles for passing into target cells is $.........:$
- A
- B
- ✓
- D
Chemical mediated gene transfer
View full question & answer→MCQ 431 Mark
Hybridoma technology was developed by:
AnswerCorrect option: D. Milstein and Kohier$, 1975$
Hybridoma technology is a technology of forming hybrid cell lines $($called hybridomas$).$
View full question & answer→MCQ 441 Mark
Which of the following bacteria is not a source of restriction endonuclease?
AnswerEntamoeba coli is not a bacterium and Haemophilus influenzae, Escherichia coli and Bacillius quifacieus are source of restriction endonuclease.
View full question & answer→MCQ 451 Mark
Which is obtained from genetic engineering.
MCQ 461 Mark
In a genetic engineering experiment, restriciton enzymes can be used for:
- A
Bacterial $\text{DNA}$ only
- B
Viral $\text{DNA}$ only
- ✓
Any $\text{DNA}$ fragment
- D
Eukaryotic $\text{DNA}$ only
AnswerCorrect option: C. Any $\text{DNA}$ fragment
View full question & answer→MCQ 471 Mark
Insect$-$resistant transgenic cotton has been produced by inserting a piece of $\text{DNA}$ from?
- A
- ✓
- C
A wild relative of cotton
- D
View full question & answer→MCQ 481 Mark
The site in the vector which helps in identifying and eliminating non transformants:
MCQ 491 Mark
$\text{pDNA}$ is used in biotechnology of $..........:$
- ✓
- B
- C
Residual $\text{DNA}$
- D
Recombinant $\text{DNA}$
View full question & answer→MCQ 501 Mark
In reference to $\text{DNA}$ polymerase $\text{III},$ which statement is wrong?
- A
It requires $\text{ATP}$ For polymerase action.
- ✓
Required for $\text{PCR}$
- C
More active than $\text{DNA}$ Pol $I\ \& \ II$
- D
Requires a previously made template to work on
AnswerCorrect option: B. Required for $\text{PCR}$
View full question & answer→