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103 questions · 63 auto-graded MCQ + 40 self-marked written.

MCQ 11 Mark
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because $–$
  • A
    Bacteria are resistant to the toxin.
  • B
    Toxin is immature.
  • Toxin is inactive.
  • D
    Bacteria encloses toxin in a special sac.
Answer
Correct option: C.
Toxin is inactive.
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Question 21 Mark
Find out from internet what is golden rice.
Answer
Golden rice is transgenic rice having gene coding for vitamin A synthesis enzyme. Golden rice was developed by Swiss Federal Institute of Technology, rich in vitamin A (beta carotene). The rice grains are golden yellow in colour due to colour it gets from the beta carotene.
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MCQ 31 Mark
Which of the following is a term used for the specific substance whose presence is being quantitatively or qualitative analysed?
  • A
    Substrate
  • Analyte
  • C
    Antibody
  • D
    None of the above
Answer
Correct option: B.
Analyte
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Question 41 Mark
How are tobacco plants benefitted when nematode specific genes are introduced into them using certain vectors? Name the vector used.
Answer
  • The tobacco plants show resistance to nematode attack; the nematode cannot survive in such plants, as they show $\text{RNA}$ interference.
  • The vector used is Agrobacterium.
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MCQ 51 Mark
The site of production of $\text{ADA}$ in the body is:
  • A
    Bone marrow.
  • Lymphocytes.
  • C
    Blood plasma.
  • D
    Monocytes.
Answer
Correct option: B.
Lymphocytes.
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Question 61 Mark
Using _______ vectors, nematode-specific genes are introduced into host plants.
Answer
Using agrobacterium vectors, nematode-specific genes are introduced into host plants.
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Question 71 Mark
What is Chakravarthy bug? Give its scientific name and its application?
Answer
Chakravarthy bug is a super bug of Pseudomonas with multiple plasmids. They are helpful in removing oil spills.
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MCQ 81 Mark
What is true about Bt toxin?
  • A
    The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.
  • B
    The concerned Bacillus has antitoxins.
  • The inactive protoxin gets converted into active form in the insect gut.
  • D
    Bt protein exists as active toxin in the Bacillus.
Answer
Correct option: C.
The inactive protoxin gets converted into active form in the insect gut.
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MCQ 91 Mark
The $\text{GEAC}$ refers to:
  • A
    Genetic Engineering Authorised Company.
  • Genetic Engineering Approval Committee.
  • C
    Genetic Engineering American Company.
  • D
    Genetic Elements and Corrections.
Answer
Correct option: B.
Genetic Engineering Approval Committee.
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MCQ 101 Mark
Restriction endonuclease is employed for cutting.
  • Double strand $\text{DNA.}$
  • B
    $\text{RNA}$ fragment.
  • C
    $\text{mRNA}.$
  • D
    A single strand $\text{DNA}.$
Answer
Correct option: A.
Double strand $\text{DNA.}$
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Question 111 Mark
Mention the chemical change that proinsulin undergoes, to be able to act as mature insulin.
Answer
The proinsulin is cleaved to remove extra stretch called the C-peptide to form mature insulin having only A-chain and B-chain joined by disulphide bond.
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Question 121 Mark
What is chimeric DNA?
Answer
Chimeric DNA molecules are produced by inserting a foreign segment of DNA into the DNA molecule of a vector.
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MCQ 131 Mark
Transgenic plants are:
  • A
    Produced by a somatic embryo in an artificial medium.
  • Generated by introducing foreign $\text{DNA}$ into a cell and regenerating a plant form.
  • C
    Produced after protoplast fusion in an artificial medium.
  • D
    Grown in an artificial medium after hybridization in the field.
Answer
Correct option: B.
Generated by introducing foreign $\text{DNA}$ into a cell and regenerating a plant form.
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MCQ 141 Mark
The royal botanical garden took $.........$ tree from Brazil:
  • A
    Neem
  • B
    Turmeric
  • Rubber
  • D
    All of the above
Answer
Correct option: C.
Rubber
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MCQ 151 Mark
'Cry’ 'protein’ coded by gene Cry $\text{IAb}$ controls:
  • A
    Cotton bollworm.
  • B
    Corn borer.
  • C
    Tobacco budworm.
  • D
    Mosquito.
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MCQ 161 Mark
The nematode that infests and damages tobacco roots:
  • A
    Ascaris megalocephala.
  • B
    Ancylostoma.
  • Meloidegyne incognitia.
  • D
    Trichinella.
Answer
Correct option: C.
Meloidegyne incognitia.
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MCQ 171 Mark
Abnormal gene is replaced by normal genes through:
  • Gene therapy
  • B
    Medicines
  • C
    Cloning
  • D
    Radiation
Answer
Correct option: A.
Gene therapy
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MCQ 181 Mark
Two statements are given$-$one labelled Assertion and the other labelled Reason. Select the correct answer to these questions from the codes $(a), (b), (c)$ and $(d)$ as given below.
Assertion: Biopatents are awarded for biological entities and all products derived from them.
Reason: Patent on use of turmeric in wound healing was cancelled in $2008.$
  • A
    Both assertion and reason are true and reason is the correct explanation of assertion.
  • B
    Both assertion and reason are true but reason is not the correct explanation of assertion.
  • Assertion is true but reason is false.
  • D
    Both assertion and reason are false.
Answer
Correct option: C.
Assertion is true but reason is false.
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MCQ 191 Mark
In genetics engineering which is used for transfer of genes from one cell to another.
  • A
    Virus
  • B
    Probe
  • C
    Plasmid
  • Vector
Answer
Correct option: D.
Vector
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MCQ 201 Mark
What will happen if we use sodium nitrate excessively in the soil?
  • Soil becomes excessively alkaline.
  • B
    Soil becomes excessively acidic.
  • C
    Soil becomes more fertile.
  • D
    Soil becomes neutral in $pH.$
Answer
Correct option: A.
Soil becomes excessively alkaline.
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MCQ 211 Mark
$\text{ELISA}$ technique is based on the principle of:
  • A
    $\text{DNA}$ replication.
  • Antigen and Antibody interaction.
  • C
    Pathogen and Antigen interaction.
  • D
    Antigen and Protein interaction.
Answer
Correct option: B.
Antigen and Antibody interaction.
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MCQ 221 Mark
Maturation of proinsulin into insulin takes place after:
  • A
    Joining of $c-$peptide.
  • B
    Addition of disulphide bridge.
  • C
    Joining of $c-$peptide.
  • Maturation of proinsulin into insulin takes place after.
Answer
Correct option: D.
Maturation of proinsulin into insulin takes place after.
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MCQ 231 Mark
A probe which is a molecule used to locate specific sequences in a mixture of $\text{DNA}$ or $\text{RNA}$ molecules could be:
  • A
    Can be $\text{ss DNA}$ but not $\text{ss RNA}.$
  • Either $\text{RNA}$ or $\text{DNA}.$
  • C
    A single stranded $\text{DNA}.$
  • D
    A single stranded $\text{RNA}.$
Answer
Correct option: B.
Either $\text{RNA}$ or $\text{DNA}.$
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MCQ 241 Mark
In some children, $\text{ADA}$ deficiency can be cured by:
  • A
    Bone marrow transplantation.
  • B
    Enzyme replacement therapy.
  • Both $(a)$ and $(b).$
  • D
    None of the above.
Answer
Correct option: C.
Both $(a)$ and $(b).$
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MCQ 251 Mark
The first successfully cloned mammals $($ani$-$ mal$)$ that gained worldwide publicity was:
  • A
    Molly $($a sheep$).$
  • B
    Polly $($a sheep$).$
  • C
    Chance $($a bull$).$
  • Dolly $($a sheep$).$
Answer
Correct option: D.
Dolly $($a sheep$).$
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Question 261 Mark
What is Bt cotton?
Answer
It is a transgenic variety of cotton which contains a foreign gene obtained from bacterium Bacillus thuringiensis.
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Question 271 Mark
State the principle on which ELISA works.
Answer
ELISA works on the principle of antigen-antibody interaction.
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MCQ 281 Mark
How does proinsulin become mature?
  • A
    By adding a short peptide.
  • By removal of $C-$peptide.
  • C
    By cutting into $2$ halves.
  • D
    By changing its $3D$ shape.
Answer
Correct option: B.
By removal of $C-$peptide.
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Question 291 Mark
What is legally wrong in the US patent law? Mention the common items of biopiracy.
Answer
US patent law does not recognise technologies and methods in use to other countries as ‘prior past’. The common items of biopiracy are soil micro-organisms, plant, animals and their genetic materials.
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MCQ 301 Mark
Gene therapy has been successful in curing genetic diseases in laboratory animals through:
  • A
    Exposure to $X-$ray to rectify the defective gene
  • Replacing the defective gene with a functional gene
  • C
    Oral delivery of genes
  • D
    Use of therapeutic medicines to rectify the defective gene
Answer
Correct option: B.
Replacing the defective gene with a functional gene
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MCQ 311 Mark
The first human hormone produced by re$-$combinant $\text{DNA}$ technology is:
  • Insulin
  • B
    Estrogen
  • C
    Thyroxine
  • D
    Progesterone
Answer
Correct option: A.
Insulin
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Question 321 Mark
Mention the problems when we consume GM food.
Answer
When we consume GM food, following problems arise:
  1. GM food may cause toxicity.
  2. The bacteria present in the alimentary canal of human could take up the antibiotic resistant gene which is present in GM food, that will cause problem.
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MCQ 331 Mark
Which of the following enzyme is used in $\text{ELISA}?$
  • A
    Horseradish peroxidase $\text{(HRP)}$
  • B
    Alkaline phosphatase
  • C
    Hexokinase
  • Both $A$ and $B$
Answer
Correct option: D.
Both $A$ and $B$
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MCQ 341 Mark
Bacillus thuringiensis $(Bt)$ strains have been used for designing novel.
  • A
    Bio$-$fertilizers.
  • B
    Bio$-$metallurgical techniques.
  • C
    Bio$-$mineralization processes.
  • Bio$-$insecticidal plants.
Answer
Correct option: D.
Bio$-$insecticidal plants.
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Question 351 Mark
Why are yeasts used extensively for functional expression of eukaryotic genes?
Answer
Yeasts are simplest unicellular eukaryotic organisms and like bacteria they are genetically well characterised, easy to grow and manipulate. They can be readily cultured in small culture vessels as well as in large-scale bioreactors.
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Question 361 Mark
Why is the gene encoding for ‘Cry’ protein inserted into a crop plant?
Answer
Cry protein producing gene is transferred to the plant to provide resistance against insect larvae.
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MCQ 371 Mark
Which is a genetically modified crop?
  • A
    Golden rice.
  • B
    $Bt-$brinjal.
  • C
    $Bt-$cotton.
  • All of these.
Answer
Correct option: D.
All of these.
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MCQ 381 Mark
The first clinical gene therapy was done for the treatment of:
  • A
    Cystic fibrosis.
  • B
    Cancer.
  • C
    $\text{AIDS}.$
  • $\text{SCID} ($Servere Combined Immuno Deficiency resulting form deficiency of $\text{ADA)}.$
Answer
Correct option: D.
$\text{SCID} ($Servere Combined Immuno Deficiency resulting form deficiency of $\text{ADA)}.$
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Question 391 Mark
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
Answer
Some Children ADA deficiency can be cured by bone marrow transplantation, in others it can be treated by enzyme replacement therapy, in which functional ADA IS given to the patient by injection.
But the problem with both of these approaches that they are not completely curative.
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Question 401 Mark
How many amino acids are arranged in the two chains of insulin?
Answer
A molecule of insulin consists of two short polypeptide chains A and B. The chain A has 21 amino acids and chain B has 30 amino acids.
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MCQ 411 Mark
A probe which is a molecule used to locate specific sequences in a mixture of $\text{DNA}$ or $\text{RNA}$ molecules could be:
  • A
    A single stranded $\text{RNA}$.
  • B
    A single stranded $\text{DNA}$.
  • Either $\text{RNA}$ or $\text{DNA}$.
  • D
    Can be $\text{ss RNA}$ but not $\text{ss RNA}$.
Answer
Correct option: C.
Either $\text{RNA}$ or $\text{DNA}$.
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Question 421 Mark
How does dsRNA gain entry into eukaryotic cell to cause RNA interference?
Answer
  1. Infection by virus having RNA genome.
  2. Mobile genetic elements (transposons) that replicate via an RNA intermediate.
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Question 431 Mark
Name the cry genes that control cotton bollworm and cotton borer, respectively.
Answer
cry IAc and cry IIAb control cotton bollworm and cry I Ab-controls corn borer.
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Question 441 Mark
Read the following and answer any four questions from (i) to (v) given below:
Golden rice was engineered from normal rice by Potrykus and Beyer in the 1990s. The typical golden colour is due to the production of $\beta$-carotene a precursor of vitamin-A. Golden rice differs from its parental strain by the addition of three $\beta$-carotene genes. These included two genes from daffodil plant and third from a bacterium. The incorporation of these genes allows the rice plant to modify certain metabolic pathways in its cells to produce $\beta$-carotene.
  1. Due to genetic modification golden rice plants produce and store $\beta$-carotene in:
  1. Stem.
  2. Seed.
  3. Leaves.
  4. All of these.
  1. Transfer of genes to produce golden rice is achieved by:
  1. Agrobacterium.
  2. pBR322
  3. $\lambda$-phage.
  4. gene gun.
  1. In golden rice two genes were taken from:
  1. Narcissus sp.
  2. Erwinia.
  3. Coryza saliva.
  4. None of these.
  1. Golden rice is helpful to fight against disease caused by the deficiency of:
  1. Vitamin $B_{12}$
  2. Vitamin C
  3. Vitamin A
  4. Vitamin D
  1. Golden rice was genetically engineered by
  1. Fire and Mello.
  2. Potrykus and Beyer.
  3. Banting and Best.
  4. Kohler and Milstein.
Answer
  1. (b) Seed.
Explanation:

Golden rice is a transgenic variety of rice (Oryza saliva) which contains good quantities of $\beta$-carotene (provitamin A - inactive state of vitamin A). $\beta$-carotene is a principal source of vitamin A. Since the grains (seeds) of the rice are yellow in colour due to $\beta$-carotene, the rice is commonly called golden rice.
  1. (a) Agrobacterium.
Explanation:

Foreign genes were inserted into the genome of a temperate rice variety by using Agrobacteriurn tumejaciens as the vector.
  1. (a) Narcissus sp.
Explanation:

ln golden rice, two genes for $\beta$-carotene were taken from daffodil (Narcissus sp.) and one gene from a bacterium Erwinia.
  1. (c) Vitamin A
Explanation:

Golden rice is rich in -carotene which is a precursor of vitamin A.
  1. (b) Potrykus and Beyer.
Explanation:

Prof. In go Potrykus and Peter Beyer produced genetically engineered rice by introducing three gene associated with synthesis of carotene. The grains (seeds) of transgenic rice are rich in provitamin.
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Question 451 Mark
What are cry genes? In which organisms are they present?
Answer
cry genes are the genes found in 'Bacillus thuringiensis' a bacterium. These genes encode for protein crystals that contain a toxic insecticidal protein called Bt toxin.
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MCQ 471 Mark
What is searched from the patient's blood in $\text{ELISA}$ method for the detection of $\text{AIDS}?$
  • A
    Antigen
  • Antibody
  • C
    No. of $\text{WBC}$
  • D
    No. of $\text{RBC}$
Answer
Correct option: B.
Antibody
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Question 481 Mark
_______ is the compound obtained from transgenic animal that is used to treat emphysema.
Answer
$\alpha\text{-1-antitrypsin}$ is the compound obtained from transgenic animal that is used to treat emphysema.
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MCQ 491 Mark
The trigger for activation of toxin of Bacillus thuringiensis is:
  • A
    Acidic $pH$ of stomach.
  • B
    High temperature.
  • Alkaline $pH$ of gut.
  • D
    Mechanical action in the insect gut.
Answer
Correct option: C.
Alkaline $pH$ of gut.
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MCQ 501 Mark
Genetically engineered bacteria have been used in commercial production of:
  • A
    Thyroxin
  • B
    Testosterone
  • Human insulin
  • D
    Melatonin
Answer
Correct option: C.
Human insulin
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MCQ 511 Mark
Insulin has $51$ amino acids arranged in $...........:$
  • A
    Single polypeptide
  • Two polypeptides of $21$ and $30$ amino acids
  • C
    Two polypeptides of $25$ and $26$ amino acids
  • D
    Three polypeptides having $15, 16$ and $20$ amino acids
Answer
Correct option: B.
Two polypeptides of $21$ and $30$ amino acids
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MCQ 521 Mark
Two statements are given$-$one labelled Assertion and the other labelled Reason. Select the correct answer to these questions from the codes $(a), (b), (c)$ and $(d)$ as given below.
Assertion: $\text{PCR}$ is routinely used for early diagnosis of $\text{HIV}$ in suspected $\text{AIDS}$ patients.
Reason: $\text{PCR}$ can detect low amounts of $\text{DNA}.$
  • A
    Both assertion and reason are true and reason is the correct explanation of assertion.
  • Both assertion and reason are true but reason is not the correct explanation of assertion.
  • C
    Assertion is true but reason is false.
  • D
    Both assertion and reason are false.
Answer
Correct option: B.
Both assertion and reason are true but reason is not the correct explanation of assertion.
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MCQ 541 Mark
Clot formation can be prevented by treatment with $...........$ in gene therapy:
  • A
    $\text{DNA}$ ase
  • B
    Recombinant vaccine
  • $\text{TPA}$
  • D
    $\text{TGF-B}$
Answer
Correct option: C.
$\text{TPA}$
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MCQ 551 Mark
Fearing that the child to be born may have a genetic disorder, a couple goes to a doctor. Which one of the following techniques is likely to be suggested by the doctor to cure the genetic disorder?
  • A
    Hybridoma technology
  • Gene therapy
  • C
    $\text{RDNA}$ technology
  • D
    Embryo transfer
Answer
Correct option: B.
Gene therapy
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MCQ 561 Mark
$\text{GEAC}$ stands for:
  • A
    Genome Engineering Action Committee.
  • B
    Ground Environment Action Committee.
  • Genetic Engineering Approval Committee.
  • D
    Genetic and Environment Approval committee.
Answer
Correct option: C.
Genetic Engineering Approval Committee.
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MCQ 571 Mark
Flavr Savr tomato is genetically modified:
  • A
    To reduce its flowering
  • To reduce its ripening rate
  • C
    To reduce its fruiting rate
  • D
    None of the above
Answer
Correct option: B.
To reduce its ripening rate
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MCQ 581 Mark
Read the following four statements $(A-D)$ about certain mistakes in two of them.
  1. The first transgenic buffalo, Rosie pro$-$duced milk which was human alpha$-$lac$-$talbumin enriched.
  2. Restriction enzymes are used in isolation of $\text{DNA}$ from other macro molecules.
  3. Downstream processing is one of the steps of $\text{R-DNA}$ technology.
  4. Disarmed pathogen vectors are also used in transfer of $\text{R-DNA}$ into the host.
Which are the two statements having mis$-$takes?
  • Statements $(A)$ and $(B)$
  • B
    Statements $(B)$ and $(C)$
  • C
    Statements $(C)$ and $(D)$
  • D
    Statements $(A)$ and $(C)$
Answer
Correct option: A.
Statements $(A)$ and $(B)$
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Question 591 Mark
What is $\alpha\text{-1-antitrypsin}?$
Answer
It is a recombinant protein produced in transgenic animals; it is used to cure emphysema.
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Question 601 Mark
In insulin molecule, which bond joins chains A and B together?
Answer
Disulfide bond joins chains A and B of insulin.
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MCQ 611 Mark
The illegal and unlawful development of biomaterials without payment to the inhabitants of their origin is called:
  • A
    Biopatent
  • B
    Biotechnology
  • C
    Bio$-$war
  • Biopiracy
Answer
Correct option: D.
Biopiracy
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MCQ 621 Mark
$C-$peptide of human insulin is:
  • A
    A part of mature insulin molecule.
  • B
    Responsible for formation of disulphide bridges.
  • Removed during maturation of pro$-$insulin to insulin.
  • D
    Responsible for its biological activity.
Answer
Correct option: C.
Removed during maturation of pro$-$insulin to insulin.
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MCQ 631 Mark
Which of the following country is principle patenting pirate?
  • A
    $\text{USA}$
  • B
    Japan
  • C
    Germany
  • All of the above
Answer
Correct option: D.
All of the above
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MCQ 641 Mark
Expand $\text{ELISA:}$
  • A
    Enzyme linked inductive assay
  • B
    Enzyme linked ion sorbent assay
  • Enzyme linked immunosorbent assay
  • D
    None of the above
Answer
Correct option: C.
Enzyme linked immunosorbent assay
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Question 651 Mark
How is biotechnology useful in increased insulin production to benefit needy people?
Answer
Biotechnology is helpful by producing genetically engineered bacteria which are capable of producing insulin in culture.
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Question 671 Mark
Read the following and answer any four questions from (i) to (v) given below:
One approach of gene therapy to treat cystic fibrosis uses viruses to deliver normal alleles of the CFTR gene into epithelial cells of the airways. A team of researchers in the USA developed a new strain of non-pathogenic adeno-associated virus (AAV), AAV2.ST. Genes for the CFTR protein and the enzyme luciferase were inserted into the DNA of the viruses. Luciferase catalyses the production of a green fluorescent protein when luciferin is added. The normal AAV strain and the AAV2.5T strain were added to cultures of epithelial cells from the airways. After adding luciferin, the number of cells that had taken up the viral genes was estimated using the intensity of the green fluorescence which developed. The result are shown in the given graph.
  1. What could be the probable reason for inserting a gene for luciferase by researchers into the viral DNA?
  1. Infected cells are able to produce luciferase.
  2. It is easy to identify the infected cells that have taken up viral DNA under fluorescent lamp.
  3. THe non-infected cells were easily identified under fluorescent lamp as they will glow.
  4. Both (a) and (b).
  1. Select the incorrect statement with respect to the graph given.
  1. Both AAV and AAV2.ST can infect epithelial cells.
  2. Intensity of green fluorescence increases more in AAV 2.5 T as compared to normal AAV.
  3. AA V infect cells more readily than AAV 2.5 T.
  4. None of these.
  1. The name of first transgenic cow is:
  1. cDNA does not contain non-coding regions such as intrans.
  2. cDNA can be transcribed and translated directly.
  3. There is no need of post-trancriptional modification such as splicing, etc.
  4. All of these.
  1. There is a decrease in intensity of green fluorescence in cells infected with AAV 2.ST during the last 10 day. This is because:
  1. Green fluorescent protein was broken down.
  2. Luciferin was used up.
  3. Infected cells die.
  4. All of these.
  1. Which of the following best describe gene therapy?
  1. Mutating the sequence of a particular gene.
  2. Replacing a healthy gene with a defective gene.
  3. Replacing a defective gene with a functional gene.
  4. Transferring a healthy gene from one species with heathy gene of other species.
Answer
  1. (d) Both (a) and (b).
Explanation:

Luciferase catalyses the production of a green fluorescent protein when luciferin is added.
  1. (c) AA V infect cells more readily than AAV 2.5 T.
Explanation:

AAV 2.5T infects cells more readily than AAV.
  1. (d) All of these.
Explanation:

cDNA is a DNA copy produced from the m RNA by reverse transcriptase.
  1. (d) All of these.
  1. (c) Replacing a defective gene with a functional gene.
Explanation:

Gene therapy refers to the insertion of genes into an individual cells and tissues to treat a disease. Essentially defective/mutant genes are replaced with healthy, functional ones.
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MCQ 681 Mark
Which of the following are not produced as transgenic animals?
  • A
    Sheep and Pig
  • B
    Rat and Rabbit
  • Dog and Banded Krait
  • D
    Cow and Fish
Answer
Correct option: C.
Dog and Banded Krait
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MCQ 691 Mark
Enzyme adenosine deaminase is deficient in $..........$ genetic disorder:
  • A
    Alzheimers disease
  • B
    Muscular dystrophy
  • $\text{SCID}$
  • D
    Colour blindness
Answer
Correct option: C.
$\text{SCID}$
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MCQ 701 Mark
In $\text{RNAi,}$ genes are silenced using.
  • A
    $\text{ss RNA}$
  • B
    $\text{ds DNA}$
  • $\text{ds RNA}$
  • D
    $\text{ss DNA}$
Answer
Correct option: C.
$\text{ds RNA}$
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MCQ 711 Mark
Which among the following is true for $BT$ toxin?
  • A
    It kills the lepidopterans.
  • B
    It is coded by 'cry' gene.
  • C
    It exists in bacteria as prototoxin.
  • All are true.
Answer
Correct option: D.
All are true.
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MCQ 721 Mark
The transgenic animals are those which have $............:$
  • A
    Foreign $\text{DNA}$ in some of its cells
  • Foreign $\text{DNA}$ in all its cells
  • C
    Foreign $\text{DNA}$ and $\text{RNA}$ in some of its cells
  • D
    Foreign $\text{DNA}$ and $\text{RNA}$ in all its cells
Answer
Correct option: B.
Foreign $\text{DNA}$ in all its cells
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Question 751 Mark
How transgenic animals, that produce useful products, can be created?
Answer
Transgenic animals that produce useful biological products can be created by introduction of the portion of DNA (for genes) which codes for a desired product.
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Question 771 Mark
How is a mature, functional insulin hormone different from its pro-hormone form?
Answer
Mature functional insulin is obtained by processing of pro-hormone which contains extra peptide called C-peptide. This C-peptide is removed during maturation of pro-insulin to insulin.
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Question 781 Mark
Name the protein produced in transgenic animals that is used to treat emphysema.
Answer
$\alpha\text{-l-antitrypsin.}$
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MCQ 791 Mark
Directions: In the following questions, the Assertions $(A)$ and Reason$(s) (R)$ have been put forward. Read both the statements carefully and choose the correct alternative from the following:
Assertion: $\text{PCR}$ can detect very low amounts of $\text{DNA}$ and is now routinely used to defect $\text{HIV}$ in suspected $\text{AIDS}$ patient.
Reason: $\text{PCR}$ is a powerful technique to identify many other genetic disorders.
  • A
    Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
  • Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
  • C
    Assertion is correct but Reason is incorrect.
  • D
    Both Assertion and Reason are incorrect.
Answer
Correct option: B.
Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
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MCQ 801 Mark
Which of the following is the application of $\text{ELISA}?$
  • A
    Detection of Mycobacterium antibodies in tuberculosis.
  • B
    Detection of rotavirus in feces.
  • C
    Detection of hepatitis $B$ markers in serum.
  • All of the above
Answer
Correct option: D.
All of the above
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Question 811 Mark
Expand $\text{GEAC}$ and $\text{ELISA}.$
Answer
  • Genetic Engineering Approval Committee.
  • Enzyme Linked Immunosorbent Assay.
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MCQ 821 Mark
Two statements are given$-$one labelled Assertion and the other labelled Reason. Select the correct answer to these questions from the codes $(a), (b), (c)$ and $(d)$ as given below.
Assertion: $GM$ foods are facing widespread resistance by the people.
Reason: $GM$ foods have mutated genes which cause infections and allergies.
  • Both assertion and reason are true and reason is the correct explanation of assertion.
  • B
    Both assertion and reason are true but reason is not the correct explanation of assertion.
  • C
    Assertion is true but reason is false.
  • D
    Both assertion and reason are false.
Answer
Correct option: A.
Both assertion and reason are true and reason is the correct explanation of assertion.
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MCQ 831 Mark
The resistant variety of rice to bacterial blight is:
  • Jaya
  • B
    $\text{TMK}-6$
  • C
    $\text{IR}-20$
  • D
    $\text{IR}-8$
Answer
Correct option: A.
Jaya
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MCQ 841 Mark
Which of the following is the key principle of bioethics?
  • A
    Animal rights
  • B
    Environmental ethics
  • C
    Autonomy
  • All of the above
Answer
Correct option: D.
All of the above
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Question 851 Mark
_______ of human insulin is removed during maturation process.
Answer
C-peptide of human insulin is removed during maturation process.
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MCQ 861 Mark
Golden rice is:
  • A transgenic rice having gene for $-$ carotene.
  • B
    Wild variety of rice with yellow coloured grains.
  • C
    Long stored rice having yellow colour tint.
  • D
    A variety of rice grown along the yellow river in China.
Answer
Correct option: A.
A transgenic rice having gene for $-$ carotene.
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MCQ 881 Mark
Choose the correct option regarding Retrovirus.
  • A
    A $\text{DNA}$ virus that can synthesise $\text{RNA}$ during infection.
  • An $\text{RNA}$ virus that can synthesise $\text{DNA}$ during infection.
  • C
    A $\text{ssDNA}$ virus.
  • D
    A $\text{dsRNA}$ virus.
Answer
Correct option: B.
An $\text{RNA}$ virus that can synthesise $\text{DNA}$ during infection.
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MCQ 891 Mark
Which kind of therapy was given in $1990$ to a four year old girl with adenosine deaminase deficiency?
  • A
    Radiation therapy
  • Gene therapy
  • C
    Chemotherapy
  • D
    Immunotherapy
Answer
Correct option: B.
Gene therapy
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MCQ 901 Mark
What are commonly called 'mobile genetic elements?
  • A
    Plasmids.
  • $\text{Tr CRNA}.$
  • C
    $\text{RNA}.$
  • D
    $\text{VNTRS}$
Answer
Correct option: B.
$\text{Tr CRNA}.$
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MCQ 911 Mark
Which of the following is the ethics for the research with animals?
  • A
    Surgical procedure under appropriate anesthesia
  • B
    Research with professional standard
  • C
    Any animal can be used
  • Both $A$ and $B$
Answer
Correct option: D.
Both $A$ and $B$
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Question 931 Mark
State the role of C-peptide in human insulin.
Answer
C-peptide (extra stretch of polypeptide) which makes the insulin inactive/proinsulin is inactive because it contain C-peptide.
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MCQ 941 Mark
Two statements are given$-$one labelled Assertion and the other labelled Reason. Select the correct answer to these questions from the codes $(a), (b), (c)$ and $(d)$ as given below.
Assertion: Organs of pig such as heart, pancreas, etc., for human use can be grown through transgenic animals.
Reason: Transgenic pigs show improved growth and meat production.
  • A
    Both assertion and reason are true and reason is the correct explanation of assertion.
  • Both assertion and reason are true but reason is not the correct explanation of assertion.
  • C
    Assertion is true but reason is false.
  • D
    Both assertion and reason are false.
Answer
Correct option: B.
Both assertion and reason are true but reason is not the correct explanation of assertion.
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MCQ 951 Mark
The competitive indirect $\text{ELISA}$ differs from direct $\text{ELISA},$ as in competitive indirect $\text{ELISA}:$
  • Unlabeled antibody is incubated in the presence of its antigen.
  • B
    Labelled antibody is incubated in the presence of its antigen.
  • C
    Labelled antigen is incubated in the presence of its antibody.
  • D
    None of the above.
Answer
Correct option: A.
Unlabeled antibody is incubated in the presence of its antigen.
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MCQ 961 Mark
Genetic engineering has been successfully used for producing.
  • A
    Transgenic mice for testing safety of polio vaccine before use in humans.
  • Transgenic models for studying new treatments for certain cardiac diseases.
  • C
    Transgenic Cow$-$Rosie which produces high fat milk for making ghee.
  • D
    Animals like bulls for farm work as they have super power.
Answer
Correct option: B.
Transgenic models for studying new treatments for certain cardiac diseases.
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MCQ 971 Mark
Using a single template molecule, how many $\text{DNA}$ molecules are generated after $10$ cycles of amplification in $\text{PCR}?$
  • $1024$ molecules.
  • B
    $1128$ molecules.
  • C
    $1224$ molecules.
  • D
    $927$ molecules.
Answer
Correct option: A.
$1024$ molecules.
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MCQ 981 Mark
$\text{PCR}$ technique was invented by:
  • A
    Boyer.
  • B
    Sanger.
  • Karry Mullis.
  • D
    None of these.
Answer
Correct option: C.
Karry Mullis.
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MCQ 991 Mark
Which of the following phenomena can be related to $\text{ELISA}?$
  • Agglutination
  • B
    Clotting
  • C
    Neutralization
  • D
    None of the above
Answer
Correct option: A.
Agglutination
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Question 1001 Mark
CryIAb is introduced in a plant to control infestation by corn borer.
  1. Name the resultant plant after successful insertion of the gene desired.
  2. Summarise the action of the gene introduced.
Answer
  1. Bt corn.
  2. CryIAb/ Bt toxin gene codes for crystal protein; the Bt toxin protein exists as an inactive protein, but once an insect ingests it, it gets converted into an active form due to the alkaline pH of the gut which solubilises the crystal. The activated toxin binds to the surface of mid gut and creates pores that cause swelling, lysis and eventually death of the insect.
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MCQ 1011 Mark
Biopiracy is related to
  • A
    Traditional knowledge
  • B
    Biomolecules and bio resources
  • C
    Isolated genes from bio resources
  • All of the above
Answer
Correct option: D.
All of the above
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MCQ 1021 Mark
The genetically$-$modified $(GM)$ brinjal in India has been developed for:
  • Insect$-$resistance.
  • B
    Enhancing shelf life.
  • C
    Enhancing mineral content.
  • D
    Drought$-$resistance.
Answer
Correct option: A.
Insect$-$resistance.
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Question 1031 Mark
Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells?
Answer
Animal cells do not have cellulose cell walls; (hence do not need the enzyme), plant cell have cellulose in their cell wall; (hence need enzyme to break it down).
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