BIOLOGY 3 Marks Question

Draw a diagram of E.coli cloning vector pBR322.

Draw the named diagram of sparged stirred tank bioreacter.

Can you recall meiosis and indicate at what stage a recombinant DNA is made?

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks.

What would be the molar concentration of human DNA in a human cell? Consult your teacher.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

find out how to distinguish between: Plasmid DNA and chromosomal DNA

Explain : PCR

(i) EcoRI has played a very important role in recombinant DNA technology.
(a) Explain the naming convention of Eco RI.
(b) Write the name of the recognition site and cleavage site of this restriction enzyme.
(ii) What is the longitudinal extension of DNA produced by these restriction enzymes called? Describe their role in the formation of r-DNA.

What is a bioreactor? Explain its functioning.

What is genetic engineering? Explain the technique of Recombinant DNA production.

Write a note on the vector relationship for gene cloning in plants and animals.

(a) Describe the role of selectable marker in r-DNA technology.
(b) Write the name of one such selectable marker considered useful for E. coli.
(c) Write one reason why it is considered an advantageous selectable marker

What is recombinant DNA? Describe the micro injection and gene gun methods of introducing foreign DNA into host cells.

Compare polymerase chain reaction and gene cloning.