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BIOLOGY M.C.Q (0.5 Marks)

Biotechnology: Principles and Processes · Rajasthan Board (RBSE) STD 12 Science (Rajasthan - English Medium). 33 questions.

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M.C.Q (0.5 Marks)

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33 questions · 32 auto-graded MCQ + 1 self-marked written.

MCQ 21 Mark
Enzyme which works by cutting DNA into segments:
  • ✓
    DNA ligase
  • B
    Restriction enzyme
  • C
    DNA polymerase
  • D
    Protease enzyme
Answer
Correct option: A.
DNA ligase
(A)DNA ligase
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MCQ 41 Mark
The following statements describe the characteristics of restriction endonuclease enzyme. Choose the wrong statement:
  • A
    This enzyme identifies a specific palindromic nucleotide sequence on DNA.
  • B
    This enzyme on DNA at the identified location, cuts the DNA molecule.
  • ✓
    This enzyme joins the DNA at specific sites and cuts only one of the two strands.
  • D
    This enzyme cuts the sugar phosphate cord at specific sites on each strand.
Answer
Correct option: C.
This enzyme joins the DNA at specific sites and cuts only one of the two strands.
(C)This enzyme joins the DNA at specific sites and cuts only one of the two strands.
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MCQ 51 Mark
Which technique is used to isolate the cut pieces of DNA by restriction endonuclease technique?
  • A
    Bioreactor
  • ✓
    Electrophoresis
  • C
    Biologic
  • D
    Microinjection
Answer
Correct option: B.
Electrophoresis
(B)Electrophoresis
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MCQ 61 Mark
Circular DNA found in bacterial cells in addition to chromosomal DNA is called:
  • A
    Episome
  • B
    Cosmid
  • ✓
    Plasmid
  • D
    Phagemid
Answer
Correct option: C.
Plasmid
(C)Plasmid
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MCQ 71 Mark
The molecule that delivers the desired DNA to the host cell is:
  • ✓
    Vector
  • B
    Host
  • C
    Parasite
  • D
    Enzyme
Answer
Correct option: A.
Vector
(A)Vector
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MCQ 81 Mark
The enzymes which cut DNA at specific recognition sites are:
  • A
    Ligase
  • B
    Exonuclease
  • ✓
    Restriction endonuclease
  • D
    DNA polymerase
Answer
Correct option: C.
Restriction endonuclease
(C)Restriction endonuclease
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MCQ 91 Mark
The basic step in genetic modification of an organism is:
  • A
    Identification of DNA containing the desired gene
  • B
    Transfer of marked DNA into host
  • C
    To preserve the transferred DNA in the host and transfer it to its progeny
  • ✓
    All of the above
Answer
Correct option: D.
All of the above
(D)All of the above
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MCQ 101 Mark
Biotechnology is part of the following:
  • A
    Synthesis and use of genes
  • B
    DNA Vaccine manufacturing or disease free
  • C
    Creation of test tube baby by in vitro fertilization
  • ✓
    All of the above
Answer
Correct option: D.
All of the above
(D)All of the above
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MCQ 111 Mark
Which of the following is used in gene cloning?
  • ✓
    Lomasosomes
  • B
    Mesosomes
  • C
    Plasmids
  • D
    Nucleoids
Answer
Correct option: A.
Lomasosomes
(A)Lomasosomes
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MCQ 121 Mark
What was used to obtain streptomycin resistant isolates in Lederberg's replica plating experiments?
  • ✓
    Minimal medium and streptomycin
  • B
    Complete medium and streptomycin
  • C
    Only minimum medium
  • D
    Only minimum medium
Answer
Correct option: A.
Minimal medium and streptomycin
(A)Minimal medium and streptomycin
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MCQ 131 Mark
Which one of the following is not a characteristic of a plasmid?
  • A
    Transferable
  • ✓
    Single-strand
  • C
    Independent replication
  • D
    Circular structure
Answer
Correct option: B.
Single-strand
(B)Single-strand
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MCQ 141 Mark
What are stirred tank bioreactors designed for?
  • ✓
    To maintain availability of oxygen during the entire process
  • B
    To maintain anaerobic conditions in the growth tube
  • C
    For purification of products
  • D
    To add preservatives to products
Answer
Correct option: A.
To maintain availability of oxygen during the entire process
(A)To maintain availability of oxygen during the entire process
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MCQ 151 Mark
Which enzymes fragment DNA?
  • A
    Ligase
  • ✓
    Type II restriction endonuclease
  • C
    Polymerase
  • D
    All of the above
Answer
Correct option: B.
Type II restriction endonuclease
(B)Type II restriction endonuclease
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MCQ 161 Mark
The method by which recombinant DNA can be injected directly into the nucleus of an animal cell is:
  • A
    Gene gun
  • B
    Biolistic
  • ✓
    Microinjection
  • D
    Bioreactor
Answer
Correct option: C.
Microinjection
(C)Microinjection
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MCQ 171 Mark
The pBR322 vector carries resistance genes to what?
  • A
    Ampicillin
  • B
    Tetracycline
  • ✓
    Both of the above
  • D
    None of the above
Answer
Correct option: C.
Both of the above
(C)Both of the above
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MCQ 181 Mark
A restriction endonuclease is named EcoRI. What does the part "co" stand for?
  • A
    Colon
  • B
    Coelom (body cavity)
  • C
    Coenzyme
  • ✓
    Coli
Answer
Correct option: D.
Coli
(D)Coli
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MCQ 191 Mark
Which disease does retrovirus cause in normal animal cells?
  • ✓
    Cancer
  • B
    Disintegration
  • C
    Tumor
  • D
    Nothing
Answer
Correct option: A.
Cancer
(A)Cancer
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MCQ 201 Mark
Which was the first restriction endonuclease identified?
  • A
    EcoRI
  • ✓
    Price II
  • C
    Hind III
  • D
    Taq I
Answer
Correct option: B.
Price II
(B)Price II
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MCQ 211 Mark
Plasmid commonly used for gene transfer in dicotyledons is:
  • A
    pBR322
  • B
    pBR235
  • ✓
    Ti Plasmid
  • D
    Cosmid
Answer
Correct option: C.
Ti Plasmid
(C)Ti Plasmid
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MCQ 221 Mark
What are the so-called 'sticky heads' made of?
  • ✓
    From unpaired bases
  • B
    With sticky polysaccharide at the end of DNA strand
  • C
    From methyl group
  • D
    From unpaired RNA bases
Answer
Correct option: A.
From unpaired bases
(A)From unpaired bases
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MCQ 231 Mark
Gene manipulation means
  • A
    Adding genetic material
  • B
    Removal of genetic material
  • C
    Fixing genetic material
  • ✓
    All of the above
Answer
Correct option: D.
All of the above
(D)All of the above
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MCQ 241 Mark
Which bacteria is more useful for gene cloning in higher plants?
  • A
    E. coli
  • B
    Rhizobium
  • C
    Agrobacterium
  • ✓
    Pseudomonas
Answer
Correct option: D.
Pseudomonas
(D)Agrobacterium
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MCQ 251 Mark
What is a single strand of nucleic acid to which a radioactive molecule has been attached called?
  • A
    Vector
  • B
    Selective marker
  • C
    Plasmid
  • ✓
    Probe
Answer
Correct option: D.
Probe
(D)Probe
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MCQ 271 Mark
Plasmid in which the desired DNA fragment is attached, is called:
  • A
    Heterogeneous plasmid
  • ✓
    Chimeric plasmid
  • C
    Celera plasmid
  • D
    Cleavable plasmid
Answer
Correct option: B.
Chimeric plasmid
(B)Chimeric plasmid
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MCQ 281 Mark
What disease does the DNA fragment (T-DNA) of Agrobacterium tumefaciens cause in normal plant cells?
  • A
    Cancer
  • B
    Decomposition
  • ✓
    Tumor
  • D
    Nothing
Answer
Correct option: C.
Tumor
(C)Tumor
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MCQ 291 Mark
At which of the following places does the restriction enzyme EcoRI cut DNA?
  • ✓
    GATTC
  • B
    GACCTG
  • C
    AAATTT
  • D
    GGGCCC
Answer
Correct option: A.
GATTC
(A)GATTC
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MCQ 301 Mark
Which of the following is not a component of downstream processing?
  • A
    Protection
  • ✓
    Expression
  • C
    Separation
  • D
    Purification
Answer
Correct option: B.
Expression
(B)Expression
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MCQ 311 Mark
Which of the following can be the desired DNA?
  • A
    Synthesized DNA
  • B
    C-DNA
  • C
    Genomic DNA
  • ✓
    All of these
Answer
Correct option: D.
All of these
(D)All of these
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MCQ 321 Mark
Restriction enzymes are found naturally:
  • A
    In eukaryotic cells
  • B
    In yeast
  • ✓
    In bacteria
  • D
    All of the above
Answer
Correct option: C.
In bacteria
(C)In bacteria
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MCQ 331 Mark
Which of the following is a restriction endonuclease?
  • A
    DNase I
  • B
    Rnase
  • ✓
    Hind II
  • D
    Protease
Answer
Correct option: C.
Hind II
(C)Hind II
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