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BIOLOGY M.C.Q (0.5 Marks)

Biotechnology—Principles and Processes · Rajasthan Board (RBSE) STD 12 Science (Rajasthan - English Medium). 57 questions.

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M.C.Q (0.5 Marks)

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57 questions · auto-graded multiple-choice test.

MCQ 11 Mark
The taq polymerase enzyme is obtained from :
  • ✓
    Thermus aquaticus
  • B
    Thiobacillus ferroxidance
  • C
    Bacillys subtilis
  • D
    Pseudomonas putida
Answer
Correct option: A.
Thermus aquaticus
A
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MCQ 21 Mark
Which of the following is a restriction enzyme endonuclease?
  • ✓
    Hind-III
  • B
    Protease
  • C
    DNAs-I
  • D
    RNAs
Answer
Correct option: A.
Hind-III
A
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MCQ 31 Mark
A molecular technique in which multiple copy of the desired gene is synthesized in-vitro is called as :
  • A
    ELISA
  • ✓
    PCR
  • C
    Gell-Electrophoresis
  • D
    Flow cytometry
Answer
Correct option: B.
PCR
B
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MCQ 41 Mark
If a foregin DNA is inserted in tetracycline resistant gene, the recombinant plasmid :
  • ✓
    Will lose ampicillin resistance
  • B
    Will lose tetracycline resistance
  • C
    Cloning would become easier
  • D
    Ampicillin resistance will become stronger
Answer
Correct option: A.
Will lose ampicillin resistance
A
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MCQ 51 Mark
Which of the following is absent in pBR322?
  • A
    Origin of replication
  • B
    Restriction site
  • ✓
    T-DNA
  • D
    Antibiotic resistant genes
Answer
Correct option: C.
T-DNA
C
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MCQ 61 Mark
For isolation of DNA a fungus is treated with which enzyme?
  • A
    Lysozyme
  • B
    Cellulase
  • ✓
    DNase
  • D
    Chitinase
Answer
Correct option: C.
DNase
C
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MCQ 71 Mark
Which of the following methods is used to inject recombinant DNA into nucleus of animal cell directly?
  • A
    Transfection
  • B
    Transformation
  • C
    Gene gun
  • ✓
    Microinjection
Answer
Correct option: D.
Microinjection
D
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MCQ 81 Mark
Which of the following is not a cloning vector for bacteria?
  • ✓
    Bacteriophage
  • B
    Plasmid
  • C
    pBR322
  • D
    T-DNA
Answer
Correct option: A.
Bacteriophage
A
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MCQ 91 Mark
Reason for movement of DNA fragments towards anode in electrophoresis is DNA fragments being :
  • A
    Negatively charged
  • ✓
    Positively charged
  • C
    Charged
  • D
    Charged with charges of both types
Answer
Correct option: B.
Positively charged
B
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MCQ 101 Mark
RNA interference is utilized to prevent nematode infestation in tobacco plants. By which vector are nematode specific genes introduced?
  • A
    Bacteriophage
  • B
    Retrovirus
  • C
    Escherichia coli
  • ✓
    Agrobacterium
Answer
Correct option: D.
Agrobacterium
D
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MCQ 121 Mark
Reagent used in ELISA test is :
  • A
    Polymerase
  • ✓
    Peroxidase
  • C
    Ligase
  • D
    Endonuclease
Answer
Correct option: B.
Peroxidase
B
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MCQ 131 Mark
Satellite DNA is a useful tool of :
  • A
    Sex determination
  • B
    Organ culture
  • ✓
    Forensic science
  • D
    Tissue culture
Answer
Correct option: C.
Forensic science
C
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MCQ 141 Mark
The DNA fragments separated on an agarose gel can be visualised after staining with :
  • A
    Aniline blue
  • ✓
    Ethidium bromide
  • C
    Bromophenol blue
  • D
    Acetocarmine
Answer
Correct option: B.
Ethidium bromide
B
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MCQ 151 Mark
A tumour inducing plasmid widely used in the production of transgenic plants is that of :
  • A
    Bacillus thuringiensis
  • ✓
    Agrobacterium tumefaciens
  • C
    Staphylococcus aureus
  • D
    E. coli
Answer
Correct option: B.
Agrobacterium tumefaciens
B
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MCQ 161 Mark
Somaclonal variations are obtained through :
  • ✓
    Tissue culture
  • B
    Gamma rays
  • C
    Amphimixis
  • D
    Chemical mutagens
Answer
Correct option: A.
Tissue culture
A
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MCQ 171 Mark
Which of the following is not a restriction enzyme?
  • A
    Eco RI
  • B
    Bam HI
  • C
    Hind III
  • ✓
    Pectinase
Answer
Correct option: D.
Pectinase
D
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MCQ 181 Mark
Enzyme required for polymerase chain reaction is :
  • A
    Endonuclease
  • B
    RNA polymerase
  • C
    Ribonuclease
  • ✓
    Taq Polymerase
Answer
Correct option: D.
Taq Polymerase
D
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MCQ 191 Mark
Nucleotide arrangement in DNA can be seen by
  • A
    Electron microscope
  • ✓
    X-ray Crystallography
  • C
    Light Microscope
  • D
    Ultracentrifuge
Answer
Correct option: B.
X-ray Crystallography
B
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MCQ 201 Mark
Which of the following restriction enzymes produces blunt ends?
  • A
    Sal I
  • ✓
    Eco RV
  • C
    Hind III
  • D
    Xho I
Answer
Correct option: B.
Eco RV
B
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MCQ 211 Mark
Which Vector can clone only a small fragment of DNA?
  • A
    Cosmid
  • B
    BAC
  • ✓
    Plasmid
  • D
    YAC
Answer
Correct option: C.
Plasmid
C
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MCQ 221 Mark
During PCR technique the pairing primers to ss DNA segment is called?
  • A
    Isolation
  • ✓
    Annealing
  • C
    Denaturation
  • D
    Polymerization
Answer
Correct option: B.
Annealing
B
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MCQ 241 Mark
Ti-plasmid is found in :
  • A
    Agrobacterium tumefaciens
  • ✓
    Agrobacterium rhizogenes
  • C
    Both (a) and (b)
  • D
    Bacillus subtilis
Answer
Correct option: B.
Agrobacterium rhizogenes
B
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MCQ 261 Mark
'GAATTC' is recognition site of which of the following?
  • ✓
    ECO-RI
  • B
    EcoR-II
  • C
    Hind-III
  • D
    BAMS-I
Answer
Correct option: A.
ECO-RI
A
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MCQ 271 Mark
Restriction enzymes are :
  • A
    Exonuclease
  • ✓
    Endonuclease
  • C
    Ligase
  • D
    Polymerase
Answer
Correct option: B.
Endonuclease
B
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MCQ 281 Mark
Restriction enzymes are known as :
  • A
    Biological guns
  • ✓
    Molecular scissors
  • C
    Plasmids
  • D
    Micropipette
Answer
Correct option: B.
Molecular scissors
B
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MCQ 301 Mark
The specific sequence of DNA recognized by Restriction Endonucleases are
  • ✓
    Palindromic Nucleotide sequence
  • B
    Anti-sense RNA
  • C
    Both (a) and (b)
  • D
    None of these
Answer
Correct option: A.
Palindromic Nucleotide sequence
A
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MCQ 321 Mark
Control of gene expression takes place at the level of :
  • ✓
    Tanscription
  • B
    Translation
  • C
    DNA replication
  • D
    Both (a) and (b)
Answer
Correct option: A.
Tanscription
A
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MCQ 331 Mark
Nucleic acid is fragmented by which enzyme?
  • A
    Polymerases
  • ✓
    Nucleases
  • C
    Proteases
  • D
    Ligases
Answer
Correct option: B.
Nucleases
B
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MCQ 341 Mark
In Ti-plasmid, which of the following is removed?
  • ✓
    Auxin gene
  • B
    Virulent gene
  • C
    Cytokinin gene
  • D
    Ausin and Cytokinin gene
Answer
Correct option: A.
Auxin gene
A
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MCQ 351 Mark
Hairy root disease in dicot plants is caused by?
  • A
    Agrobacterium tumefaciens
  • ✓
    Agrobacterium rhizogens
  • C
    Bacillus thuringiensis
  • D
    Meloidogyne incognita
Answer
Correct option: B.
Agrobacterium rhizogens
B
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MCQ 361 Mark
Gene gun is used to :
  • A
    Cut the plasmid
  • B
    Bind the cut DNA
  • ✓
    Introduce alien DNA into host cell
  • D
    Purify DNA
Answer
Correct option: C.
Introduce alien DNA into host cell
C
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MCQ 371 Mark
Enzyme used in cutting DNA at specific site is :
  • A
    DNA ligase
  • ✓
    Restriction endonuclease
  • C
    Ribozyme
  • D
    Lysozyme
Answer
Correct option: B.
Restriction endonuclease
B
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MCQ 381 Mark
Transgenic plants are developed by :
  • ✓
    Introduction of foreign genes
  • B
    Clone and genetically modified genes
  • C
    Genetic engineering
  • D
    Purified genes
Answer
Correct option: A.
Introduction of foreign genes
A
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MCQ 391 Mark
DNA element with ability to change its position is called :
  • A
    Cistron
  • ✓
    Transposon
  • C
    Muton
  • D
    Recon
Answer
Correct option: B.
Transposon
B
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MCQ 401 Mark
Golden rice is a promising transgenic crop. When released for cultivation, it will help in?
  • A
    High lysin content
  • B
    Pest resistance
  • C
    High protein content
  • ✓
    High vitamin A content
Answer
Correct option: D.
High vitamin A content
D
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MCQ 411 Mark
Who among the following was awarded the Nobel Prize for the development of PCR technique?
  • A
    Herbert Boyer
  • B
    Hargobind Khurana
  • ✓
    Kary Mullis
  • D
    Arthur Karnberg
Answer
Correct option: C.
Kary Mullis
C
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MCQ 421 Mark
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
  • A
    Laboratory Flask of largest capacity
  • B
    A stirred-tank bioreactor without inlets and outlets
  • ✓
    A continuous culture system
  • D
    Any of the above
Answer
Correct option: C.
A continuous culture system
C
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MCQ 431 Mark
Which of the following step is catalysed by Tag polymerase in a PCR reaction?
  • A
    Denaturation of template DNA
  • B
    Anneling of primers on the template DNA
  • ✓
    Extension of primer end on the template DNA
  • D
    All of the above
Answer
Correct option: C.
Extension of primer end on the template DNA
C
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MCQ 441 Mark
Which of the following bacteria is not a source of restriction endonuclease?
  • A
    Haemophilus influenzae
  • B
    Escherichia coli
  • ✓
    Agrobacterium tumefaciens
  • D
    Bacillus amyloli
Answer
Correct option: C.
Agrobacterium tumefaciens
C
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MCQ 451 Mark
Plasmid is a :
  • A
    Fungus
  • B
    Virus
  • C
    Part of plasma membrane
  • ✓
    Extra chromosomal DNA in bacterial cell
Answer
Correct option: D.
Extra chromosomal DNA in bacterial cell
D
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MCQ 461 Mark
An antibiotic resistance gene in a vector usually helps in the selection of :
  • A
    Competent cells
  • ✓
    Transformed cells
  • C
    Recombinant cells
  • D
    None of these
Answer
Correct option: B.
Transformed cells
B
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MCQ 471 Mark
A recombinant DNA molecule can be produced in the absence of the following?
  • A
    Restriction endonuclease
  • B
    DNA Ligase
  • C
    DNA Fragments
  • ✓
    E. coli
Answer
Correct option: D.
E. coli
D
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MCQ 481 Mark
An enyme catalysing the removal of nucelotides from the ends of DNA is :
  • A
    Endonuclease
  • ✓
    Exonuclease
  • C
    DNA Ligase
  • D
    Hind-II (a)
Answer
Correct option: B.
Exonuclease
B
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MCQ 491 Mark
Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from :
  • A
    Plasmids
  • B
    All prokaryotic cells
  • C
    Bacteriophages
  • ✓
    Bacterial cell
Answer
Correct option: D.
Bacterial cell
D
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MCQ 501 Mark
The most important feature in a plasmid to be used as a vector is :
  • ✓
    Origin of replication
  • B
    Presence of selectable marker
  • C
    Site for restriction endonuclease
  • D
    Its size
Answer
Correct option: A.
Origin of replication
A
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MCQ 511 Mark
While isolating DNA from bacteria, which of the following enzyme is not used?
  • A
    Lysozyme
  • B
    Ribonuclease
  • ✓
    Deoxyribonuclease
  • D
    Protease
Answer
Correct option: C.
Deoxyribonuclease
C
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MCQ 521 Mark
In agarose gel electrophoresis, DNA molecules are separated on the basis of their?
  • A
    Charge only
  • ✓
    Size only
  • C
    Charge to size ratio
  • D
    All of these
Answer
Correct option: B.
Size only
B
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MCQ 531 Mark
The transfer of genetic material from a bacterial cell to another through the mediation a vector like virus is termed as :
  • ✓
    Transduction
  • B
    Conjugation
  • C
    Transformation
  • D
    Translation
Answer
Correct option: A.
Transduction
A
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MCQ 541 Mark
Who discovered Restricted endonuclease enzymes?
  • ✓
    Smith and Nathane
  • B
    Baiger
  • C
    Waxman
  • D
    Flemming
Answer
Correct option: A.
Smith and Nathane
A
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MCQ 551 Mark
Which enzyme custs DNA at specific sites?
  • A
    DNA polymerase
  • B
    Taq-polymerase
  • C
    Topoisomerase
  • ✓
    Restriction endonuclease
Answer
Correct option: D.
Restriction endonuclease
D
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MCQ 561 Mark
Which of the following bacteria synthesizes insecti-cidal protein?
  • A
    Agrobacterium
  • ✓
    Bacillus Thuringiensis
  • C
    Escherichia coli
  • D
    Archaebacteria
Answer
Correct option: B.
Bacillus Thuringiensis
B
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MCQ 571 Mark
From which of the following bacteria, thermostable DNA polymerase is isolated?
  • A
    Agrobacterium
  • ✓
    Thermus aquaticus
  • C
    Methanobacterium
  • D
    Archaebacteria
Answer
Correct option: B.
Thermus aquaticus
B
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