


Tissue Culture: Tissue culture is the technique in which ‘ex vivo growth of cells or tissues in an aseptic and nutrient-rich medium’ is done. While performing experiments of tissue culture, a liquid, solid or gel-like ” medium prepared from agar, is used. Such medium supplies nutrients and energy necessary for tissue culture technique. Different processes are to be done while performing tissue culture, viz. primary treatment, reproduction or multiplication, shooting and rooting, primary hardening, secondary hardening, etc. From the source plant, required tissues are taken out and all the processes are carried in an aseptic medium in laboratory.
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