MCQ
At which temperature a DNA molecule is denatured by heat in PCR:
- A70o - 80oC
- B90o - 95oC
- C42oC
- D50o - 65oC
Explanation:
Denaturation: The reaction temperature is increased to 95 degree centigrade, which melts (disrupts the hydrogen bond between complementary bases) all dsDNA into single-stranded DNA (ssDNA).
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| Column $- I$ | Column $- II$ |
| $(a)$ Endometrium | $(p)$ The most external layer |
| $(b)$ Myometrium | $(q)$ Help in collection of the ovum after ovulation |
| $(c)$ Fimbriae | $(r)$ Fertilised ovum is installed here |
| $(d)$ Perimetrium | $(s)$ Thick layer of smooth muscle |