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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Bihar BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
At which temperature a DNA molecule is denatured by heat in PCR:
  • A
    70o -  80oC
  • B
    90o - 95oC
  • C
    42oC
  • D
    50o - 65oC
✓

Answer

  1. 90o - 95oC

Explanation:

Denaturation: The reaction temperature is increased to 95 degree centigrade, which melts (disrupts the hydrogen bond between complementary bases) all dsDNA into single-stranded DNA (ssDNA).

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