Question
ATP produced during glycolysis is a result of substrate level phosphorylation. Explain.

Answer

When formation of ATP happens by direct transfer of a phosphoryl (PO3) group to ADP from a phosphorylated reactive intermediate; this is called substrate level phosphorylation. During glycolysis, ATP is formed at two stages because of direct transfer of phosphoryl group to ADP. When BPGA (biphosphoglyceric acid) is converted into PGA (phosphoglyceric acid). When PEP (phosphoenolpyruvate) is converted into pyruvic acid.

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