Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
Question
Before integrating DNA with bacterial plasmid, bacterial cells are treated with calcium. Why?
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Answer
DNA is a hydrophillic molecule which can not pass through the cell membrane, so to make it competent to take up DNA, bacterial cells should be treated with divalent cations or calcium so that DNA can enter through the pores of cell wall.
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