Question
Describe the process of paper chromatography.

Answer

Process of paper chromatography:
  • The mixture of the compound to be analysed is dissolved in a suitable solvent and spotted on the chromatography paper about 2 cm from one end of the paper using a glass capillary.
  • The paper is then suspended in a chamber containing the mobile phase.
  • The mobile phase rises up the paper and flows over the spot, due to capillary action.
  • Different solutes are retained differently on the paper depending on their selective partitioning between the two phases. The paper strip so developed, is known as chromatogram.

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