Maharashtra BoardEnglish MediumSTD 11 ScienceChemistryBasic Analytical Techniques4 Marks
Question
Describe the process of paper chromatography.
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Answer
Process of paper chromatography:
The mixture of the compound to be analysed is dissolved in a suitable solvent and spotted on the chromatography paper about 2 cm from one end of the paper using a glass capillary.
The paper is then suspended in a chamber containing the mobile phase.
The mobile phase rises up the paper and flows over the spot, due to capillary action.
Different solutes are retained differently on the paper depending on their selective partitioning between the two phases. The paper strip so developed, is known as chromatogram.
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