Cell: The Unit of Life — BIO BOTANY STD 11 — Question
Tamilnadu BoardEnglish MediumSTD 11BIO BOTANYCell: The Unit of Life5 Marks
Question
Describe the steps involved in cytologieal techniques.
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Answer
There are different types of mounting based on the portion of a specimen to be observed.
Whole mount: The whole organism or smaller structure is mounted over a slide and observed.
Squash: Is a preparation where the material to be observed is crushed/squashed onto a slide so as to reveal their contents. Example: Pollen grains, mitosis and meiosis in root tips and flower buds to observe chromosomes.
Smears: Here the specimen is in the fluid (blood and microbial cultures etc) are scraped, brushed or aspirated from surface of organ. Example: Epithelial cells.
Sections: Free hand sections from a specimen and thin sections are selected, stained and mounted on a slide. Example: Leaf and stem of plants.
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