Plant Water Relation — BIOLOGY STD 12 Science — Question

Read the following and answer any four questions from (i) to (v) given below:
Ginkgo tree has been saved from extinction by selective breeding followed by channeling into trade of nature lovers. This is an ex-situ conservation, where endangered species are protected from all adverse factors. Offspring produced in captive breeding are released in natural habitat for acclimatisation.

1. What is the significance of offsite collections?

1. Restock depleted populations.
2. Protection of endangered species.
3. Reintroduce species in wild.
4. All of these.

2. Which of the following is not an ex-situ conservation?

1. Wetlands
2. Orchards
3. Aquaria
4. Botanical gardens.

3. Select the incorrect statements for ex-situ conservation.

1. It is conservation of species outside their natural habitats.
2. Endangered species are kept under human supervision and provided all the essentials.
3. The species population recovers in natural environment.
4. Both (b) and (c).

4. Conditions maintained in seed banks for orthodox seeds are:

1. Low moisture content.
2. Anaerobic conditions.
3. Low temperature.
4. All of these.

5. Assertion: Animal species which have become extinct in wild continue to be maintained in zoological parks.

Reason: Captive breeding is the conservation of those cases where these is no realistic chance of in-situ survival.

1. Both assertion and reason are true and reason is the correct explanation of assertion.
2. Both assertion and reason are true but reason is not the correct explanation of assertion.
3. Assertion is true but reason is false.
4. Both assertion and reason are false.

Read the following and answer any four questions from (i) to (v) given below:
The foundations of recombinant DNA (rDNA) were laid by the discovery of restriction enzymes. These enzymes are present in many bacterias where they function as a part of their defense mechanism called the Restriction Modification system (RM system). Molecular basis of this system was explained first by Werner Arber in 1962. The Restriction Modification system consists of two components:

• A restriction enzyme (called restriction endonuclease) identifies the introduced foreign DNA and cuts it into pieces.
• The second component is a modification enzyme (methylase) that adds a methyl group to DNA at specific site to protect it from the restriction enzyme cleavage.

1. Restriction endonucleases are enzymes present in ________ where they function as a part of _______ mechanism.

1. (i) bacteria (ii) digestive
2. (i) protists (ii) transcription
3. (i) plant cells (ii) replication
4. (i) prokaryotes (ii) defence

2. Which of the following statements regarding modification enzyme is correct?

1. It adds methyl group to one or two bases usually within the host DNA sequence to protect it from the restriction enzyme.
2. It adds ethyl group to one or two bases usually within the sequence recognised by the restriction enzymes.
3. It adds methyl group to only one of bases within the foreign DNA sequence that is recognised by the restriction enzymes.
4. None of these.

3. Which of the following is a type II restriction enzyme?

1. Alu I
2. EcoR I
3. BamH I
4. All of these.

4. Which of the following is the first discovered restriction endonuclease?

1. Sal I
2. EcoR I
3. Hind II
4. EcoR II

5. Components of Restriction Modification System include?

1. Restriction enzyme.
2. Modification enzyme.
3. Lysing enzyme.
4. Both (a) and (b).

Read the following and answer any four questions from (i) to (v) given below:
DNA fingerprinting is a technique of determining nucleotide sequences of certain areas of DNA which are unique to each individual. Each person has a unique DNA fingerprint. Each fingerprint is the same for every cell, tissue and organ of a person. DNA fingerprinting is the basis of paternity testing in case of disputes.

1. The technique developed to identify a person with the help of DNA restriction analysis is known as.

1. DNA profiting
2. DNA fingerprinting
3. RFLP
4. Both (a) and (b).

2. For DNA fingerprinting, DNA is obtained from.

1. Blood
2. Hair root cells
3. Semen
4. All of these

3. During DNA fingerprinting, the radioactive probes.

1. Hybridise with DNA sample to form double stranded structure
2. Egrade the DNA
3. Create positive charge on DNA
4. Cut the DNA sample at various sites.

4. In India, DNA fingerprinting technique was developed by?

1. Dr. Lalji Singh
2. Alec Jeffreys
3. Dr. Khorana
4. None of these.

5. Which of the following is true about DNA fingerprinting?

1. VNTR is used as probe.
2. DNA samples are loaded on agarose gel electrophoresis.
3. It is based on identification of nucleotide sequence present on the DNA molecule.
4. All of these

Distinguish Between: Dominant characters and Recessive characters.

Read the following and answer any four questions from (i) to (v) given below:
In recombinant DNA technology, the fragments of DNA generated after cutting the DNA by restriction enzymes are separated according to their size or length by gel electrophoresis. Gel electrophoresis is performed in a gel matrix so that molecules of similar electric charges can be separated on the basis of size. Most commonly used matrix in gel electrophoresis is agarose. The fragments are separated under the influence of electric field. The separated DNA fragments can be seen only after staining the DNA with compound known as ethidium bromide (EtBr) followed by exposure to UV radiation as bright orange band.

1. Gel electrophoresis is used for the separation of

1. DNA only.
2. DNA and RNA only.
3. DNA and proteins only.
4. DNA, RNA and proteins.

2. Most commonly used matrix is _____ which is a ______ extracted from _______.

1. (i) agarose (ii) polysaccharide (iii) sea weed
2. (i) agarose (ii) protein (iii) sea weed
3. (i) EtBr (ii) polysaccharide (iii) sea weed
4. (i) EtBr (ii) protein (iii) bacteria

3. A DNA molecule was treated with a restriction endonuclease and three fragments of size (i) 426kb, (ii) 129kb and (iii) 46kb were obtained. Identify the order in which these bands will arrange themselves in the gel plate after gel electrophoresis is completed. (Assuming that negative part of electrode is towards the well)

1. (iii) → (ii) → (i)
2. (i) → (ii) → (iii)
3. (i) → (iii) → (ii)
4. (iii) → (i) → (ii)

4. Which of the following statements regarding gel electrophoresis is incorrect?

1. Separated DNA fragments can be seen only after staining DNA with EtBr.
2. DNA fragments are separated according to their size.
3. Under the influence of electric field, positively charged molecules move towards the anode and negatively charged molecules move towards the cathode.
4. None of these.

5. The factor that will not affect the rate of DNA migration in gel electrophoresis is:

1. Size of DNA molecule.
2. Concentration of DNA.
3. Voltage supplied.
4. Concentration of the gel.

Read the following and answer any four questions from (i) to (v) given below:
During copulation, semen is released by the penis into the vagina. 'The motile sperms swim rapidly, fuse with ovum in the ampullary region, resulting in fertilisation. Haploid nucleus of sperm fuse with that of ovum to form diploid zygote.

1. In female genital tract, sperms are made capable of fertilising the egg. This phenomenon of sperm activation is called:

1. Amphimixis.
2. Cortical reaction.
3. Capacitation.
4. Acrosomal reaction.

2. Select the correct sequence of various physical and chemical events that take place during fertilisation.

16. Fusion of cortical granules with plasma membrane of secondary oocyte.
17. Formation of fertilisation cone to receive sperm.
18. Release of sperm lysin from acrosome.
19. Mixing up of chromosomes of a sperm and an ovum.

1. R → Q → P → S
2. Q → S → R → P
3. Q → R → S → P
4. R → P → Q → S

3. Assertion: Only one sperm can fertilise an ovum.

Reason: The secretion of acrosome help the sperm to enter into cytoplasm of ovum through zona pellucida and plasma membrane.

1. Both assertion and reason are true, and reason is the correct explanation of assertion.
2. Both assertion and reason are true, but reason is not the correct explanation of assertion.
3. Assertion is true, but reason is false.
4. Both assertion and reason are false

4. What is the significance of fertilisation?

1. It restores haploid number of chromosomes.
2. It produces offspring genetically identical to parents.
3. It initiates cleavage.
4. Both (b) and (c).

5. Site of fertilisation in humans is:

1. Endometrium of uterine cavity.
2. Ampullary isthmic junction of oviduct.
3. Cervix of uterus.
4. Infundibulum of fallopian tube.

Distinguish between : Inborn immunity and acquired immunity.