Question
Enumerate the steps which are involved in recombinant DNA technology.

Answer

  1. Isolation of DNA.
  2. Fragmentation of DNA by restriction endonucleases.
  3. Isolation of desired DNA fragment.
  4. Ligation of DNA fragment into vector.
  5. Transferring recombinant DNA into host.
  6. Culturing host cells at large scale.
  7. Extraction of the desired product.

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