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Biotechnology — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology3 Marks
Question
Explain different types of restriction enzymes?
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Answer

Different types of restrictions enzymes are as follows:
  1. Type I – They function . simultaneously as endonuclease and methylase e.g. EcoK.
  2. Type II – They exhibit separate cleaving and methylation activities. They are more stable and are used in r-DNA technology e.g. EcoRI, Bgll. They cut DNA at specific sites within the pallindrome. Thousands of type II restriction enzymes have been discovered.
  3. Type III – They cut DNA at specific non- palindromic sequences e.g. Hpal, MboII.

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