Recognition sequence of restriction enzymes are generally ……………….. nucleotide long.
- A2 to 4
- ✓4 to 8
- C8 to 10
- D14 to 18
Answer: B.
View full solution →Question types
Biotechnology question types
387 questions across 10 question groups — pick any mix to generate a BIOLOGY paper with step-by-step answer keys.
387
Questions
10
Question groups
5
Question types
01
MCQ
70 Q→02Answer the following questions
152 Q→03Answer the following questions.
45 Q→04Answer the following questions in detail.
35 Q→05Answer the following questions in detail.
46 Q→06Complete the following table.
4 Q→07Diagram based question.
4 Q→08Write the short note.
8 Q→09Answer the following in detail :
18 Q→10Case study (4 Marks)
5 Q→Sample Questions
Biotechnology questions
One sample from each question group in this chapter. Select any group above to see the full set with answer keys.
Recombinant protein ……………….. is used to dissolve blood clots present in the body.
- Ainsulin
- ✓tissue plasminogen activator
- Crelaxin
- Derythropoietin
Answer: B.
View full solution →Which of the following is the correct recognition sequence of restriction enzyme hind III.
- ✓5′ —A-A-G-C-T-T— 3′
3′ —T-T-C-G-A-A—5′ - B5′ — G-A-A-T-T-C—3′
3′ — C-T-T-A-A-G—5′ - C5′ — C-G-A-T-T-C—3′
3′ — G-C-T-A-A-G—5′ - D5′ — G-G-C-C—3′
3′ — C-C-G-G—5′
Answer: A.
View full solution →In vitro amplification of DNA or RNA segment is known as ………………..
- Achromatography
- Bsouthern blotting
- ✓polymerase chain reaction
- Dgel electrophoresis
Answer: C.
View full solution →The enzyme nuclease hydrolyses ……………….. of polynucleotide chain of DNA.
- Ahydrogen bonds
- ✓phosphodiester bonds
- Cglycosidic bonds
- Dpeptide bonds
Answer: B.
View full solution →Fill in the blanks and complete the chart.
| GMO | Purpose |
| (i) Bt cotton | ———– |
| (ii) ———- | Delay the softening of tomato during ripening |
| (iii) Golden rice | ———– |
| (iv) Holstein cow | ———– |
Expand the following acronyms which are used in the held of biotechnology:
- YAC
- RE
- dNTP
- PCR
- GMO
- MAC
- CCMB.
Give the role of Ca++ ions in the transfer of recombinant vector into bacterial host cell.
Which cells from Langerhans of pancreas do produce a peptide hormone insulin?
Name the vector which is used in production of human insulin through recombinant DNA technology.
Fill in the blanks and complete the chart.
| GMO | Purpose |
| (i) Bt cotton | ———– |
| (ii) ———- | Delay the softening of tomato during ripening |
| (iii) Golden rice | ———– |
| (iv) Holstein cow | ———– |
Expand the following acronyms which are used in the held of biotechnology:
- YAC
- RE
- dNTP
- PCR
- GMO
- MAC
- CCMB.
Give the role of Ca++ ions in the transfer of recombinant vector into bacterial host cell.
| Recombinent protein | Its use in or for |
| (1) Platelet derived growth factor | (a) Anemia |
| (2) a-antitrypsin | (b) Cystic fibrosis |
| (3) Relaxin | (c) Haemophilia A |
| (4) Eryhthropoietin | (d) Diabetes |
| (5) Factor VIII | (e) Emphysema |
| (6) DNA ase | (f) Parturition |
| (g) Atherosclerosis |
In the process of r-DNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of r-DNA or chimeric DNA? Explain.
Discuss various health concerns regarding the use of GMO crops.
What are the adverse effects of Biotechnology on the Environment?
What is meant by ethics?
How transgenic animals are produced.?
Give any two examples of biofortified transgenic crops.
Enlist and write in brief about the different biological tools required in r-DNA technology.
Enlist different types of restriction enzymes commonly used in r-DNA technology? Write on their role.
What is a vaccine? Give advantages of oral vaccines or edible vaccines.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
How are the transgenic mice used in cancer research?
| Organization | Expand |
| OECD | —————— |
| GEAC | —————— |
| USDA | —————— |
| USPTO | —————— |
| CSIR | —————– |
| Substance | Potential benefit | Crop | Transgene |
| Provitamin A | Anti-oxidant | ————– | Phytoene synthase, Lycopene cyclase |
| Fructans | ————– | Sugarbeet | —————- |
| Vitamin E | —————- | Canola | γ -tocopherol methyl transferase |
| Flavonoids | Anti-oxident | Tomato | ————– |
| ————– | Iron fortification | Rice | Ferritin, metallothioein, phytase |
| RE | Source | End products |
| Alu I | ————- | Blunt ends |
| ———— | Bacillus amyloliquefaciens H | Sticky ends |
| Eco R I | ————– | Sticky ends |
| Hind II | H. influenza Rd | ————– |
Classify the following: (i) Provide tissues for human transplants
(ii) Cancer research
(iii) E. coli hygromycin resistant gene
(iv) Supply of factor IX.
(ii) Cancer research
(iii) E. coli hygromycin resistant gene
(iv) Supply of factor IX.
| Column A: (Transgenic animal) | Column B : (Application) |
| (a) Transgenic mice | —————— |
| (b) Transgenic cattle | —————— |
| (c) Pig clones | —————— |
| (d) Transgenic fish | —————— |
Draw a diagram showing steps in r-DNA technology.
Draw a labelled diagram of a plasmid showing ori, ampr and a region into which foreign DNA can be inserted.
(a) Which enzyme has recognition sequence shown in the diagram given below?
(b) What is meant by palindrome?
(c) Enzyme in the given diagram cuts DNA to produce ———- ends.
(b) What is meant by palindrome?
(c) Enzyme in the given diagram cuts DNA to produce ———- ends.
(a) Name the reaction shown in the given diagram.
(b) What are the three steps in this reaction?
(b) What are the three steps in this reaction?
Transgenic fish.
Transgenic cattle.
Insulin.
Golden rice.
Bt cotton.
What is meant by traditional knowledge? What is its importance?
Write any two scientific and commercial values of transgenic animals in favour of human beings.
What factors are responsible for losses during storage and transport of crops? Explain, with example, how genetic engineering can reduce these losses?
With the help of a suitable example, illustrate palindrome.
What are the different techniques and devices used in r-DNA technology?
| Restriction enzyme | Recognition sequence |
| (1) Alu I | (a) 5′ G-A-A-T-T-C 3′ 3′ C-T-T-A-A-G 5’ |
| (2) Bam HI | (b) 5′ G-T-C-G-A-C3′ 3′ C-A-G-C-T-G 5′ |
| (3) Eco RI | (c) 5′ A-G-C-T 3′ 3′ T-C-G-A5′ |
| (4) Hind II | (d) 5′ G-G-A-T-T-C 3′ 3′ C-C-T-A-A-G 5′ |
| Column A | Column B |
| (1) Sir Edward Sharpey Schafer | (a) Chemically synthesized DNA sequence of insulin |
| (2) Hakura | (b) Discovery of insulin |
| (3) Gilbert and Villokomaroff | (c) PCR |
| (4) K. Mullis | (d) Insulin production using r-DNA technology |
Germ line gene therapy and somatic cell gene therapy.
Genomic library and c-DNA library.
Old or classical biotechnology and Modem biotechnology.
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