grobacterium tumifaciens is used in gene cloning. However, Agrobacterium tumifaciens is a pathogenic pathogen of many dicotyledonous plants. It transforms normal plant cells into tumors by transferring a segment of DNA called 'T-DNA' and these tumor cells produce chemicals necessary for the pathogen. On this basis, the Ti plasmid of Agrobacterium tumifaciens has now been converted into a cloning vector, which is not pathogenic for plants, but is used to transfer genes of interest to many plants.