Question
Explain the mode of action of ECORI.
✓
Answer
- The restriction enzyme, ECORI recognises the palindromic sequence, shown below:
- It cuts the DNA strands a little away from the centre of the palindrome site, but between the same two bases on both the strands (indicated by the arrows)
- This leaves single-stranded stretches, called sticky ends, overhanging at the end of the strands.
- DNA ligase can catalyse hydrogen bond formation between the cut complementary parts of the vector DNA and foreign DNA.
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