Question
Explain with example how transformed host cells are selected from non- transformed?
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Answer
- Transformed recombinant cells are selected using marker genes.
- For example, markers genes in pBR 322 plasmid are ampicillin resistant gene and tetracyclin resistant gene.
- When pstl restriction enzyme is used, ampicillin resistant gene gets knocked off from the plasmid and recombinant cells become sensitive to ampicillin.
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