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Biotechnology — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology3 Marks
Question
Explain with example how transformed host cells are selected from non- transformed?
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Answer

  1. Transformed recombinant cells are selected using marker genes.
  2. For example, markers genes in pBR 322 plasmid are ampicillin resistant gene and tetracyclin resistant gene.
  3. When pstl restriction enzyme is used, ampicillin resistant gene gets knocked off from the plasmid and recombinant cells become sensitive to ampicillin.

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