Question
- If a desired gene is identified in an organism for some experiments explain the process of cutting this desired gene at specific location.
- Mention the function of stirrer in a bioreactor.
✓
Answer
- The process of cutting the desired gene (also called restriction digestion) is carried out by incubating the purified DNA molecules with the specific restriction enzyme at optimal conditions of pH, temperature etc. of the enzyme.
- The stirrer facilitates the even mixing and oxygen availability throughout the contents in the reactor.
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