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$I.$ DNA being a hydrophilic molecule cannot pass through cell membranes
$II.$ Agrobacteriumtumefaciens delivers a piece of $DNA$ known as $'Z-DNA'$ in the Tiplasmid which transforms normal plant cells into tumour cells to produce chemical against pathogens
$III.$ Retrovirus, adenovirus, papillomavirus are also now used as cloning vectors in animal because of their ability to transform normal cells into cancerous cell.
$IV.$ In genetic engineering, $DNA$ from different sources are cut with the same restriction enzymes so that both $DNA$ fragments have same kind of sticky ends
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