Question
List in proper sequence the processes involved in recombinant DNA (rDNA) technology.
✓
Answer
The steps are:
- Isolation of DNA.
- Fragmentation of DNA by restriction endonucleases.
- Isolation of a desired DNA fragment.
- Amplification of the desired DNA fragment.
- Ligation of the DNA fragment into a vector.
- Transferring the recombinant DNA into the host.
- Culturing the host cells and extraction of the desired gene product on a large scale.
- Downstream processing, i.e. separation and purification.
Need a full question paper?
Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.
Explore more
Similar questions
Draw a well labelled diagram of mature graafian follicle.
Who propounded the Central Dogma principle and what is meant by it?
Which three options could be thought for increasing food production?
Mention three physical properties and three chemical properties of soil, that determine the type of vegetation in a given region.
Nikhil goes with his family for dinner and insists on sitting in the non-smoking area to which his father (who is a heavy smoker) obiects.
The following is the flowchart highlighting the steps in DNA fingerprinting technique. Identify A, B, C, D, E and F.
Refer to the figure given below and answer the questions that follow:
- Recognize and explain the process by which Tasmanian wolf evolved.
- Give one example of an animal that has evolved along with Tasmanian wolf.
- Compare and contrast the two animals shown?
"The codon is a triplet and is read in a contiguous manner without punctuations." Provide the genetic basis for the statement.
Explain the experiment done by Miller regarding the origin and development of life. ###Make a clearly labeled picture of Stanley Miller's experiment. ###How the experiment done by Miller proved that the initial development of life happened from preexisting lifeless organic molecules.
Draw a labelled diagram of biogas plant.