Question
Manish while performing a practical in laboratory on Grams stain observed that one type of bacteria stained dark blue or purple in colour, while the other type of bacteria did not retain the crystal violet dye even after staining again and again. Instead it keeps on loosing the dye on washing. He rushed to his teacher for showing the specimen.

His teacher explained that there is nothing to get worried about. As different kinds ofbacteria are found.

  1. What is the basis of classifying bacteria.
  2. What is the basic difference between Gram positive and Gram negative bacteria.
  3. Name one Gram positive and one Gram negative bacteria.
  4. What values are shown by Manish's teacher?

Answer

  1. Bacteria are classified into two groups on the basis of the differences in the cell envelopes and the manner in which they respond to Gram stain. Staining of bacteria with Gram stain revealed the basis to differentiate bacteria into two categories, i.e., Gram positive and Gram negative bacteria.
  2. The basic difference between the two types of bacteria lies in their cell wall. The Gram positive bacteria contains peptidoglycan and teichoic acid in their cell wall while, the cell wall of Gram negative bacteria is not composed of peptidoglycan (in high amount), instead they have an outer layer made up of lipids. They also do not contain teichoic acid.
  3. Gram positive Clostridium and Gram negative Acetobacter.
  4. Values shown by Manish's teacher are awareness about types of bacteria, critical thinking, patience, understanding and right attitude.

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