MCQ
Polyethylene glycol method is used for :
  • A
    Seedless fruit production
  • B
    Energy production from sewage
  • Gene transfer without a vector
  • D
    Biodiesel production

Answer

Correct option: C.
Gene transfer without a vector
Polyethylene glycol $\text{(PEG)}$ is a polyether compound.
The compound has varied applications. Its biological uses include being used as a precipitant for plasmid $\text{DNA}$  isolation and protein crystallisation. $\text{PEG}$ is also used as a fusing agent in the transformation experiments.
The gene or the desired fragment of $\text{DNA}$ can be transferred into the host cell without the use of vector. $\text{PEG}$ with calcium ions allows the membrane to become charged and allow the entry of the gene.

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