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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
Polyethylene glycol method is used for:
  • A
    Seedless fruit production
  • B
    Energy production from sewage
  • C
    Gene transfer without a vector
  • D
    Biodiesel production
✓

Answer

  1. Gene transfer without a vector

Explanation:

Polyethylene glycol (PEG) is a polyether compound. The compound has varied applications. Its biological uses include being used as a precipitant for plasmid DNA isolation and protein crystallisation. PEG is also used as a fusing agent in the transformation experiments. The gene or the desired fragment of DNA can be transferred into the host cell without the use of vector. PEG with calcium ions allows the membrane to become charged and allow the entry of the gene.

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