$A$. Substrate enzyme complex formation.
$B$ Free enzyme ready to bind with another substrate.
$C$. Release of products.
$D$. Chemical bonds of the substrate broken.
$E$. Substrate binding to active site.
Choose the correct answer from the options given below :
The catalytic cycle of an enzyme action can be described in the following steps.
($1$) The binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate.
($2$) The active site of the enzyme, now in close proximity of the substrate breaks the chemical bonds of the substrate and the new enzyme-product complex is formed.
($3$) The enzyme releases the products of the reaction and the free enzyme is ready to bind to another molecule of the substrate and run through the catalytic cycle once again.
($4$) First, the substrate binds to the active site of the enzyme, fitting into the active site.
Options ($1$), ($2$) and ($3$) are incorrect as the steps mentioned are in the wrong sequence.
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$I.$ In microinjection method foreign $DNA$ is directly injected into the nucleus of animal cell or plant cell by using micro needles or micro pipettes
$II.$ Microinjection method is used in oocytes, eggs and embryo
$III.$ Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
$IV.$ In chemical mediated gene transfer method certain chemicals such as $\mathrm{CO}_{2}$ help foreign $DNA$ to enter the host cell
Which of the statements given above are correct?