MCQ
'Restriction' in Restriction enzyme refers to:
- ACleaving of phosphodiester bond in DNA by the enzyme.
- BCutting of DNA at specific position only.
- CPrevention of the multiplication of bacteriophage in bacteria.
- DAll of the above.
Restriction enzymes belong to a class of enzyme called nuclease. Restriction enzymes prevent the multiplication of bacteriophage in bacteria. They are used for cutting of DNA at specific position which becomes possible by cleaving of phosphodiester bond in DNA by the enzyme. Option ‘c’ is the correct answer.
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$I$. In the absence of adenosine deaminase enzyme, purine metabolism is disturbed and $T-$lymphocytes fails to function $II$. $ADA$ deficiency is caused by the deletion of the gene for $ADA$
$III$. In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy. But in both approaches, the patients are not completely cured
$IV$. For permanent cure, genes isolated from the bone marrow cells producing $ADA$ at early embryonic stages can be a possible cure
Which of the above statements are correct?