MCQ
The correct sequence of different steps of polymerase chain reaction is:
- AAnnealing denaturation extension
- BDenaturation extension annealing
- CDenaturation annealing extension
- DExtension denaturation annealing
Explanation:
Polymerase chain reaction is a technique used to replicate a fragment of DNA so as to produce many copies of a particular DNA sequence. A single PCR amplification cycle involves three basic steps: denaturation, annealing and extension (polymerisation).
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$I$. Lymphocytes are obtained from the patients
$II$. Lymphocytes are transferred to the culture dishes
$III$. Lymphocytes are transfected with the normal $ADA$ genes
$IV$. The transfected cell are returned to the patients
The chronological order should be