MCQ
The most important feature in a plasmid to be used as a vector is:
- AOrigin of replication (ori).
- BPresence of a selectable marker.
- CPresence of sites for restriction endonuclease.
- DIts size.
Origin of replication is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. So, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number.
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