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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
Question
Two statements are given-one labelled Assertion and the other labelled Reason. Select the correct answer to these questions from the codes (a), (b), (c) and (d) as given below.
  1. Both assertion and reason are true and reason is the correct explanation of assertion.
  2. Both assertion and reason are true but reason is not the correct explanation of assertion.
  3. Assertion is true but reason is false.
  4. Both assertion and reason are false.
Assertion: The insertion of DNA fragment into pBR 322 plasmid using enzyme Pst I or Pvu I make ampicillin resistant gene non functional.
Reason: Bacterial cells containing recombinant pBR322 is unable to grow in the presence of ampicillin.
✓

Answer

  1. Both assertion and reason are true but reason is not the correct explanation of assertion.

Explanation:

Plasmid pBR322 has a variety of unique restriction sites for restriction endonucleases. Two unique sites, Pst I andPvu /are located within the amp' gene and Bamrll, Sal I etc. are within tet1 gene. The presence of restriction sites within the marker tef and amp' permits an easy selection for cell transformed with the recombinant pBR322. Insertion of the DNA fragment into the plasmid using enzyme Pst I and Pvu I places the DNA insert within the gene amp' and make it non functional.

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