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Biotechnology — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology3 Marks
Question
What are the basic requirements of $\text{PCR}$ technique?
✓

Answer

$1.\ \text{DNA.}$ containing the desired segment to be amplified.
$2.$ Excess of forward and reverse primers which are synthetic oligonucleotides of $17$ to $30$ nucleotide.
$3.$ They are complementary to the sequences present in $\text{DNA.}$
$\text{dNTPs}$ which are of four types such as $\text{dATB dGTB dTTP}$ and $\text{dCTR}$
$4.$ A thermostable $\text{DNA}$ polymerase $($e.g. Taq $\text{DNA}$ polymerase enzyme$)$ that can withstand a high temperature of $90-98^\circ C.$
$5.$ Appropriate quantities of$ Mg^{++}$ ions.
$6.$ Thermal cycler, a device required to carry out $\text{PCR}$ reactions.

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