Question
What are the basic requirements of $ \text{PCR}$ technique?
✓
Answer
The basic requirements of $ \text{PCR}$ technique are as follows:
- $ \text{DNA}$ containing the desired segment to be amplified.
- Excess of forward and reverse primers which are synthetic oligonucleotides of $17$ to $30$ nucleotide.
- They are complementary to the sequences present in $ \text{DNA}.$
$ \text{dNTPs}$ which are of four types such as $ \text{dATB dGTB dTTP}$ and $ \text{dCTR}$ - A thermostable $ \text{DNA}$ polymerase $($e.g. Taq $ \text{DNA}$ polymerase enzyme$)$ that can withstand a high temperature of $90-98^\circ C.$
- Appropriate quantities of $Mg^{++}$ ions.
- Thermal cycler, a device required to carry out $ \text{PCR}$ reactions.
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