Question
What are the different techniques and devices used in r-DNA technology?
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Answer
(1) Several techniques are used in r-DNA technology to isolate and characterize the macromolecules like DNA, RNA, proteins.
(2) The techniques used on the basis of molecular weight are gel permeation, osmotic pressure, ion exchange chromatography, spectroscopy, mass spectrometry, electrophoresis, etc.
(3) Electrophoresis:
- It is used for the separation of charged molecules like DNA, RNA and proteins, by application of an electric field.
- Different types of electrophoresis : Agarose gel electrophoresis, PAGE, SDA PAGE.
(4) Polymerase chain reaction (PCR) : It is used for in vitro gene cloning or gene multiplication to produce a billion copies of the desired segment of DNA or RNA, with high accuracy and specificity, in few hours.
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