Question
What is pBR322? Describe it by drawing a picture.
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Answer
This plasmid is used as a cloning vector. In this p = Plasmid, BR = the first letter of the name of the scientists Bolivar and Rodrigan who created it and 322 of their experiments. Their size can range from 1.5 Kb to 1500 Kb. It can contain anywhere from three genes to several thousand genes. Only DNA fragments smaller than 15 Kb can be cloned in this plasmid (see Fig. 9.5).
Two antibiotic resistance genes are found in it. One gene confers resistance to ampicillin and the other to tetracycline. Recognition sites of many specific restriction enzymes are found in it. 4361 base pairs are found in it and it is obtained in pure form.
Two antibiotic resistance genes are found in it. One gene confers resistance to ampicillin and the other to tetracycline. Recognition sites of many specific restriction enzymes are found in it. 4361 base pairs are found in it and it is obtained in pure form.
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