Question
Which methodology is used while sequencing the total DNA from a cell? Explain it in detail.
✓
Answer
Methodology used:
- Sequence Annotation-total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors.
- The cloning resulted into amplification of each piece of DNA fragment.
- The fragments were sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping regions (present in them).
- This requires generation of overlapping fragments (for sequencing).
- Specialised computer based programmes were developed, and these sequences were subsequently annotated and assigned to each chromosome.
Need a full question paper?
Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.
Explore more
Similar questions
What are recombinant DNA vaccines? Give two examples of such vaccines. Discuss their advantages.
Why ozone hole forms over Antarctica? How will enhanced ultraviolet radiation affect us?
The graph shows species-area relationship:
- If b denotes the relationship on log scale-
- Describe a and b.
- How is slope represented? Give the normal range of slope.
- What kind of slope will be observed for frugivorous birds and mammals in a tropical forest?
- Species diversity of plants (22%) is much less than that of animals (72%). Analyze the reasons for greater diversity of animals as compared to plants.
What is post-fertilization event? Describe the structure of plant endosperm. Also draw a well labelled diagram of dicot embryo.
Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR.
A child has blood group O. If the father has blood group A and mother blood group B, work out the genotypes of the parents and the possible genotypes of the other offsprings.
Explain the steps involved in a polypeptide synthesis. How are the amino acids activated during polypeptide synthesis?
Given below is the diagram of a human ovum surrounded by a few sperms. Observe the diagram and answer the following questions:
Compare the fate of sperms shown in the diagram.
Compare the fate of sperms shown in the diagram.
What is Pulse Polio Programme of Government of India? What is OPV? Why is it that India is yet to eradicate Polio?
Do all the gametes formed from a parent organism have the same genetic composition? Analyse the situation with the background of gametogenesis and provide suitable explanation.