Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes5 Marks
Question
Which methodology is used while sequencing the total DNA from a cell? Explain it in detail.
✓
Answer
Methodology used:
Sequence Annotation-total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors.
The cloning resulted into amplification of each piece of DNA fragment.
The fragments were sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping regions (present in them).
This requires generation of overlapping fragments (for sequencing).
Specialised computer based programmes were developed, and these sequences were subsequently annotated and assigned to each chromosome.
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