$(A)$ Denaturation of proteins causes loss of secondary and tertiary structures of the protein
$(B)$ Denturation leads to the conversion of double strand of $DNA$ into single strand
$(C)$ Denaturation affects primary structure which gets distorted
$Hb{O_2} + {H_3}{O^ + }C{O_2} \rightleftharpoons \left( {{H^ + } - Hb - C{O_2}} \right) + {O_2} + {H_2}O$
Release of $O_2$ is favoured when there is