Question
Why are vectors needed for replication of DNA during rDNA technology?

Answer

The vector provides the origin of replication.

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Similar questions

Explain Methodology of HGP
Explain in situ approach for conservation of biodiversity.
Microbes can be used to decrease the use of chemical fertilizers and pesticides. Explain how this can be accomplished.###How biofertilizers increase fertility of soil?
Read the following and answer any four questions from (i) to (v) given below:
The Kakapo is the world's largest and heaviest parrot, found only in New Zealand. It is unusual in that it is nocturnal, flightless and ground-dwelling. It is an excellent climber of trees, has strong legs that allow it to "jog" several kilometres in a single trip, and has mossy green plumage mottled with brown and yellow. The Kakapo is also critically endangered as of now, there were only few known living individuals left.
  1. Which could be the possible reason for Kakapo to be well-adapted to its environment prior to the arrival of humans in New Zealand?
  1. Kakapo was active only in the night when its potential predators would not be out for hunting.
  2. The Kakapo would likely be well-camouflaged among the forest foliage due to its greenish plumage.
  3. It was able to effectively hunt for food in the night.
  4. All of these.
  1. When humans started to settle in New Zealand, they took with them non-native animals, including mammals such as cats, dogs and stoats. By which of the following ways, human settlement likely contributed to a near decimation of Kakapo populations in New Zealand?
  1. Habitat destruction.
  2. Alien species invasion.
  3. Pollution.
  4. Both (a) and (b).
  1. All known survived Kakapo have been relocated by the New Zealand government to three predator-free islands, where they are monitored year round by staffs and volunteers to ensure that the birds are safe, healthy and well-fed. The extremely low population of Kakapo is a hurdle to the species becoming viable in the long term, despite such dedicated conservation efforts. This is because.
  1. The small population results in very small gene pool.
  2. There would be very Ii mited genetic diversity among the resulting offspring.
  3. Of reduced capacity of the species to adapt and survive changes in the environment.
  4. All of these.
  1. The reasons behind conserving biodiversity have been grouped into which of the following categories?
  1. Narrowly utilitarian.
  2. Broadly utilitarian.
  3. Ethical.
  4. All of these.
  1. One of the ex situ conservation methods for endangered species is:
  1. Wildlife sanctuaries.
  2. Biosphere reserves.
  3. Cryopreservation.
  4. National parks.
Explain bioreactor with diagram.
Read the following and answer any four questions from (i) to (v) given below:
Disposal of untreated sewage into the river or freshwater pond causes huge water pollution. Four water samples from different sources (A, B, C, D) are collected and tested for BOD value in a lab to assess their quality. The BOD values are presented in the given table. Water samples are collected from primary effluent, secondary effluent, untreated sewage and river water. Study the given table and answer the following.
Sample BOD
A 20mg/ L
B 5mg/ L
C 300mg/ L
D 400mg/ L
  1. The source of sample 'C' is:
  1. River water.
  2. Primary effluent.
  3. Secondary effluent.
  4. Untreated sewage water.
  1. If sewage in untreated condition is disposed off in a freshwater body then.
  1. BOD and dissolved oxygen both will increase.
  2. BOD will increase and dissolved oxygen will decreas.
  3. BOD will decrease and dissolved oxygen will increase.
  4. BOD and dissolved oxygen both will decrease.
  1. A large number of pathogenic microbes can be present in water sample of:
  1. C
  2. A
  3. D
  4. Both (a) and (c).
  1. High value of BOD in sample O is due to:
  1. High amount of organic wastes and aerobic microbes.
  2. High amount of inorganic wastes and anaerobic microbes.
  3. High amount of organic wastes and anaerobic microbes.
  4. High amount of inorganic wastes and aerobic microbes.
  1. River water is represented by the sample.
  1. A
  2. B
  3. C
  4. D
Read the following and answer any four questions from (i) to (v) given below:

The mature ovum or a female gamete is spherical in shape. The human ovum is almost free of yolk and is said to be alecithal. Human ovum loses its ability to be fertilised about 24 hours after ovulation. Refer to the given structure of ovum and answer the following questions.

  1. Thick cellular layer formed of radially elongated follicular cells is:
  1. Zona pellucida.
  2. Plasma membrane.
  3. Perivitelline membrane.
  4. Corona radiata.
  1. In humans, at which stage does ovum get released from ovary?
  1. Secondary oocyte.
  2. Oogonium.
  3. Primary oocyte.
  4. First polar body.
  1. Cytoplasm of an ovum is enveloped by _____.
  1. Zona pellucida.
  2. Corona radiata.
  3. Cell membrane.
  4. Perivitelline space.
  1. Select the correct option:
S. No
V
W
X
(a)
Cytoplasm
Zona pellucida
Plasma membrane
(b)
Cortical granules
Corona radiata
Zona pellucida
(c)
Cortical granules
Plasma membrane
Corona radiata
(d)
Cytoplasm
Corona radiata
Zona pellucida
  1. Which of the following is not a characteristic of an ovum?
  1. Nucleus of an ovum has prominent nucleolus.
  2. Only one ovum formed from one oogonium.
  3. It lacks centrioles.
  4. It has very small amount of ooplasm.
Explain the methods for making host cell competent to insert the r DNA.
Read the following text carefully and answer the questions that follow:
Malaria and dengue fever are major mosquito-borne public health problems in tropical countries. The authors report a malaria and dengue co-infection in an 11-year-old boy who presented with sustained fever for 10 days. The physical examination revealed a flushed face, injected conjunctivae and left submandibular lymphadenopathy. His peripheral blood smear showed few ring-form trophozoites of Plasmodium falciparum. His blood tests were positive for dengue NS-1 antigen and IgM antibody, and negative for IgG antibody. After the initiation of antimalarial treatment with artesunate and mefloquine, his clinical condition gradually improved. However, he still had low-grade fever that persisted for 6 days. Finally, he recovered well without fluid leakage, shock or severe bleeding.
i. Name the fish that help in eradication of mosquito larvae.
ii. What is the reason of symptoms of malaria?
iii. Name the body parts and host in which following events takes place in life cycle of plasmodium.
a. asexual reproduction
b. sexual reproduction.
OR
Name some vector borne diseases and their vector.
Read the following and answer any four questions from (i) to (v) given below:
The first menstruation is called menarche, that usually occurs between 12 and 15 years. In human females, menstruation is repeated at an average interval of about 28/29 days and is called menstrual cycle. It is regulated by certain hormones, as pituitary gland is stimulated by releasing factors produced in the hypothalamus. The hormones produced by pituitary gland influence the ovaries. The hormones secreted by the ovaries affect the walls of the uterus.
  1. The breakdown of endometrium is characteristic of:
  1. Proliferative phase.
  2. Luteal phase.
  3. Ovulatory phase.
  4. Menstrual phase.
  1. Which days of the menstrual cycle marks the proliferative phase?
  1. 1-5
  2. 15-28
  3. 6-13
  4. 10-14
  1. Which of the following occurs during secretory phase?
  1. Empty Graafian follicle changes into corpus luteum.
  2. Primary follicle changes into Graafian follicle.
  3. Endometrium rebuilds and estrogen secretion increases.
  4. LH surge, inducing release of an ovum.
  1. Identify the hormones that attain peak level during ovulatory phase.
  1. FSH.
  2. Progesterone.
  3. LH.
  4. Both (a) and (c).
  1. Withdrawal of which hormone causes degeneration of corpus luteum?
  1. FSH.
  2. LH.
  3. Progesterone.
  4. Estrogen.