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Biotechnology and its Applications — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology and its Applications3 Marks
Question
  1. Why must a cell be made 'competent’ in biotechnology experiments? How does calcium ion help in doing so?
  2. State the role of ‘biolistic gun’ in biotechnology experiments.
✓

Answer

  1.  
  1. To take up the (hydrophilic) DNA from the external medium.
  2. Divalent calcium ions increase the efficiency of DNA entering the cell through pores in the cell wall.
  1. To introduce alien DNA into the plant cell by bombarding them with high-velocity microparticles. (gold or tungsten coated with DNA)

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