Question
Write a detailed account on Dark field microscope.
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Answer
The dark field microscope was discovered by Z. Sigmondy (1905). Here the field will be dark but object will be glistening so the appearance will be bright. A special effect in an ordinary microscope is brought about by means of a special component called “Patch Stop Carrier”. It is fixed in metal ring of the condenser component.
Patch stop is a small glass device which has a dark patch at centre of the disc leaving a small area along the margin through which the light passes. The light passing through the margin will travel oblique like a hollow cone and strikes the object in the periphery, therefore the specimen appears glistening in a dark background.
Patch stop is a small glass device which has a dark patch at centre of the disc leaving a small area along the margin through which the light passes. The light passing through the margin will travel oblique like a hollow cone and strikes the object in the periphery, therefore the specimen appears glistening in a dark background.
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