Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
Write a short article on the utility of bacteriophage as a vector.
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Answer
In bacteriophage, generally a linear DNA molecule is found. When it is broken at one place, two pieces are formed. By insertion of the desired DNA between these two segments, recombinant bacteriophage DNA gets constructed. Bacteriophage infects bacteria and completes the lysis cycle and produces recombinant DNA. This wild bacteriophage DNA is recreated by removing the essential parts. In this recreated bacteriophage DNA, 20-25 Kb of external DNA is inserted. Many bacteriophages, mainly $\lambda$ (Lamda) and $M _{13}$ phages, are used as vectors.
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