Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
You have created a recombinant DNA molecule by ligating a gene to a plasmid vector. By mistake, your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go for transformation now?
✓

Answer

The experiment will not likely be affected as recombinant DNA molecule is circular and closed, with no free ends.
Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "Answer the following questions." from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Maharashtra Board STD 12 Science question papers→Maharashtra Board question paper generator→

Similar questions

List the four different human male accessory ducts.
→
Explain briefly:
Restriction enzymes and DNA
→
What is biogas? Why is it preferred to other conventional energy fuels?
→
What is diapause and its significance?
→
Define spermicides. Also, mention their use and mode of contraceptive action.
→
Thermal power plants are inevitable in an industrial and densely populated country like ours. What harm do they do to the environment? Also mention any precautions that could be taken to save our environment.
→
Why are the human testes located outside the abdominal cavity? Name the pouch in which they are present.
→
Give an account of incomplete dominance with a suitable example.
→
Expand the following and mention one application of each:
  1. PCR.
  2. ELISA.
→
Differentiate between menarche and menopause.
→