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BIOLOGY Case study (4 Marks)

Biotechnology · Maharashtra Board STD 12 Science (MSBSHSE - English Medium). 5 questions.

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Case study (4 Marks)

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5 questions · timed · auto-graded

Question 14 Marks
Restriction enzymeRecognition sequence
(1) Alu I(a) 5′ G-A-A-T-T-C 3′ 3′ C-T-T-A-A-G 5’
(2) Bam HI(b) 5′ G-T-C-G-A-C3′ 3′ C-A-G-C-T-G 5′
(3) Eco RI (c) 5′ A-G-C-T 3′ 3′ T-C-G-A5′
(4) Hind II(d) 5′ G-G-A-T-T-C 3′ 3′ C-C-T-A-A-G 5′
Answer
Restriction enzymeRecognition sequence
(1) Alu I(c) 5′ A-G-C-T 3′ 3′ T-C-G-A5′
(2) Bam HI(d) 5′ G-G-A-T-T-C 3′ 3′ C-C-T-A-A-G 5′
(3) Eco RI(a) 5′ G-A-A-T-T-C 3′ 3′ C-T-T-A-A-G 5’
(4) Hind II (b) 5′ G-T-C-G-A-C3′ 3′ C-A-G-C-T-G 5′
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Question 24 Marks
Column AColumn B
(1) Sir Edward Sharpey Schafer(a) Chemically synthesized DNA sequence of insulin
(2) Hakura(b) Discovery of insulin
(3) Gilbert and Villokomaroff(c) PCR
(4) K. Mullis (d) Insulin production using r-DNA technology
Answer
Column AColumn B
(1) Sir Edward Sharpey Schafer(b) Discovery of insulin
(2) Hakura(a) Chemically synthesized DNA sequence of insulin
(3) Gilbert and Villokomaroff(d) Insulin production using r-DNA technology
(4) K. Mullis (c) PCR
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Question 34 Marks
Germ line gene therapy and somatic cell gene therapy.
Answer
Germ line gene therapy Somatic cell gene therapy
1. Germ line gene therapy involves modification of genome of germ cells like sperms, eggs, early embryos.1. Somatic cell gene therapy involves modification of genome of somatic cells like bone marrow cells, hepatic cells, fibroblasts, endothelium, pulmonary epithelial cells, central nervous system and smooth muscle cells of wall of blood vessels.
2. It allows transmission of the modified genetic information to the next generation.2. It does not allow transmission of the modified genetic information to the next generation.
3. Its application in human beings is not encouraged because of technical and ethical reasons.3. It is a feasible option and clinical trials are carried out for treatment of various diseases.
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Question 44 Marks
Genomic library and c-DNA library.
Answer
Genomic libraryc-DNA library
1. It is a collection of clones that represent the complete genome of an organism.1. It is a collection of clones containing c-DNAs inserted into suitable vectors like phages or plasmids.
2. DNA fragments to be cloned are obtained by cutting genomic DNA by restriction  enzymes.2. c-DNAs are produced by the process of reverse transcription, using complete m-RNA complement obtained from a tissue or an organism.
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Question 54 Marks
Old or classical biotechnology and Modem biotechnology.
Answer
Old or classical biotechnology Modem biotechnology
1. It is mainly based on fermentation technology.1. It is based on genetic engineering and chemical engineering.
2. It does not involve alteration or modification of genetic material of organisms to develop specific Product.2. It involves the alteration or modification of genetic material of organisms to develop specific product.
3. It does not involve the use of r-DNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing to develop products.3. It involves the use of r-DNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing to develop specific products.
4. There is no ownership of old biotechnology.4. It involves ownership of technology.
5. Examples : Preparation of curd, ghee, soma, vinegar, yogurt, cheese making, wine making, etc.5. Examples : Transgenic organisms.
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