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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
A vector is engineered with three features which facilitate its cloning within the host cell. List the three features and explain each one of them.
✓

Answer

  1. Origin of replication/ ori site: From here the replication starts (and any piece of DNA when linked, can be made to replicate within the host cell).

  2. At least two Selectable markers: Helpful in identifying and eliminating non-transformants.

  3. Unique Restriction sites for more than one restriction enzymes: The foreign DNA links to this region of the plasmid.

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